Product nameAnti-CD133 antibody
See all CD133 primary antibodies
DescriptionRabbit polyclonal to CD133
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IHC-P, ICC, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: Human Embryonic Stem Cell Lysate; MEL-1, HT29 and Caco-2 whole cell lysates. IHC-P: Human kidney and glioblastoma multiforme brain tissues; Mouse and rat kidney tissues. ICC/IF: Mouse neural stem cells; Caco-2 cells. Flow Cyt: KM-H2 cells.
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab19898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/50 - 1/200. PubMed: 23769181|
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 97 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionMay play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Tissue specificityIsoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).
Involvement in diseaseRetinitis pigmentosa 41
Cone-rod dystrophy 12
Stargardt disease 4
Retinal macular dystrophy 2
Sequence similaritiesBelongs to the prominin family.
modificationsIsoform 1 and isoform 2 are glycosylated.
Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
Cellular localizationApical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.
- Information by UniProt
- AC133 antibody
- Antigen AC133 antibody
- CD133 antibody
Image courtesy of Human Protein Atlas
ab19898 staining CD133 in human kidney. Paraffin embedded human kidney was incubated with ab19898 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6
ab19898 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Sample: mouse neural stem cells, isolated from the brain of E14 mouse embryo. IF was performed the day after isolation. The antibody was used in concentration 1:200, and was equally effective at the concentration 1:500.
CD133 staining is shown in red; DAPI staining of nuclei is shown in blue.
All lanes : Anti-CD133 antibody (ab19898) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Human Embryonic Stem Cell Lysate with CD133 peptide (ab20651) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 97 kDa
Anti-CD133 antibody ab19898 detects a band corresponding to the expected size of CD133 in Human Embryonic Stem Cell Lysate using Western Blotting. It is likely that the band runs higher than the predicted MW of CD133 due to glycosylation of CD133. In mouse neural stem cell and mouse embryonic stem cell lysate this ~110 kDa band was not detected but a reproducible band of ~30 kDa was seen. This could represent a cleavage product but it may also be that the ~30 kDa band represents non-specific binding by ab19898. However, ab19898 has been shown to recognise mouse neural stem cells using ICC. Blocking using the immunising peptide removed both of these bands.
ab19898 at 1/200 staining mouse kidney tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed and the tissue was then incubated with ab19898 for 16 hours. An Alexa-Fluor ® 488conjugated goat anti-rabbit antibody was used as the secondary.
ab19898 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in formaldehyde buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
All lanes : Anti-CD133 antibody (ab19898) at 1 µg/ml
Lane 1 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 2 :
HT29 whole cell lysate (ab3952)
Lane 3 :
Caco 2 whole cell lysate (ab3950)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
ab19898 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Asynchronous KM-H2 cells were pelleted and labeled by indirect immunofluorescence. Cells were stained with ab19898 (1/200) for 30min at 4'C, washed and then stained with goat anti-rabbit alexafluor 488 (1/200). Forward/Side scatter were used to eliminate cellular debris. The accompanying marker was applied such that only 2% of the IgG control was positive. Based on the accompanying image, approximately 48% of cells exhibited positive staining for anti-CD133. This image is taken from an Abreview.
Paraformaldehyde-fixed, paraffin-embedded human glioblastoma multiforme brain tissue stained for CD133 using ab19898 at 1/100 dilution in immunohistochemical analysis.
Formaldehyde-fixed, 0.1% Triton-BSA permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells stained for CD133 (red) using ab19898 at 1/50 dilution in ICC/IF, followed by Goat Anti-Rabbit IgG (H+L) Cy3. Nuclei staining with Hoechst (blue).
Formaldehyde-fixed, paraffin-embedded rat kidney tissue stained for CD133 using ab19898 at 1/1500 dilution in immunohistochemical analysis, followed by Goat Anti Rabbit IgG Biotin.
This product has been referenced in:
- Zhai R et al. Pharmacological Mobilization of Endogenous Bone Marrow Stem Cells Promotes Liver Regeneration after Extensive Liver Resection in Rats. Sci Rep 8:3587 (2018). Read more (PubMed: 29483616) »
- Zhao C et al. ETV2 mediates endothelial transdifferentiation of glioblastoma. Signal Transduct Target Ther 3:4 (2018). Read more (PubMed: 29527330) »