Overview

  • Product name
  • Description
    Rabbit polyclonal to CD133
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IHC-P, ICC, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human CD133.

    Read Abcam's proprietary immunogen policy (Peptide available as ab20651.)

  • Positive control
    • WB: Human Embryonic Stem Cell Lysate; MEL-1, HT29 and Caco-2 whole cell lysates. IHC-P: Human kidney and glioblastoma multiforme brain tissues; Mouse and rat kidney tissues. ICC/IF: Mouse neural stem cells; Caco-2 cells. Flow Cyt: KM-H2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab19898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/300.
IHC-Fr 1/50 - 1/200. PubMed: 23769181
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 97 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
  • Tissue specificity
    Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).
  • Involvement in disease
    Retinitis pigmentosa 41
    Cone-rod dystrophy 12
    Stargardt disease 4
    Retinal macular dystrophy 2
  • Sequence similarities
    Belongs to the prominin family.
  • Post-translational
    modifications
    Isoform 1 and isoform 2 are glycosylated.
    Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
  • Cellular localization
    Apical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.
  • Information by UniProt
  • Database links
  • Alternative names
    • AC133 antibody
    • Antigen AC133 antibody
    • CD133 antibody
    • CORD12 antibody
    • Hematopoietic stem cell antigen antibody
    • hProminin antibody
    • MCDR2 antibody
    • MSTP061 antibody
    • OTTHUMP00000217744 antibody
    • OTTHUMP00000217745 antibody
    • OTTHUMP00000217746 antibody
    • PROM1 antibody
    • PROM1_HUMAN antibody
    • Prominin I antibody
    • Prominin like 1 antibody
    • Prominin like protein 1 precursor antibody
    • Prominin mouse like 1 antibody
    • Prominin-1 antibody
    • Prominin-like protein 1 antibody
    • Prominin1 antibody
    • PROML1 antibody
    • RP41 antibody
    • STGD4 antibody
    see all

Images

  • Image courtesy of Human Protein Atlas

    ab19898 staining CD133 in human kidney. Paraffin embedded human kidney was incubated with ab19898 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6

    ab19898 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • Sample: mouse neural stem cells, isolated from the brain of E14 mouse embryo. IF was performed the day after isolation. The antibody was used in concentration 1:200, and was equally effective at the concentration 1:500.

    CD133 staining is shown in red; DAPI staining of nuclei is shown in blue.

  • All lanes : Anti-CD133 antibody (ab19898) at 1 µg/ml

    Lane 1 : Human Embryonic Stem Cell Lysate
    Lane 2 : Human Embryonic Stem Cell Lysate with CD133 peptide (ab20651) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 97 kDa



    Anti-CD133 antibody ab19898 detects a band corresponding to the expected size of CD133 in Human Embryonic Stem Cell Lysate using Western Blotting. It is likely that the band runs higher than the predicted MW of CD133 due to glycosylation of CD133. In mouse neural stem cell and mouse embryonic stem cell lysate this ~110 kDa band was not detected but a reproducible band of ~30 kDa was seen. This could represent a cleavage product but it may also be that the ~30 kDa band represents non-specific binding by ab19898. However, ab19898 has been shown to recognise mouse neural stem cells using ICC. Blocking using the immunising peptide removed both of these bands.

  • ab19898 at 1/200 staining mouse kidney tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed and the tissue was then incubated with ab19898 for 16 hours. An Alexa-Fluor ®  488conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab19898 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in formaldehyde buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • All lanes : Anti-CD133 antibody (ab19898) at 1 µg/ml

    Lane 1 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
    Lane 2 : HT29 whole cell lysate (ab3952)
    Lane 3 : Caco 2 whole cell lysate (ab3950)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    ab19898 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Asynchronous KM-H2 cells were pelleted and labeled by indirect immunofluorescence. Cells were stained with ab19898 (1/200) for 30min at 4'C, washed and then stained with goat anti-rabbit alexafluor 488 (1/200). Forward/Side scatter were used to eliminate cellular debris. The accompanying marker was applied such that only 2% of the IgG control was positive. Based on the accompanying image, approximately 48% of cells exhibited positive staining for anti-CD133. This image is taken from an Abreview.

  • Paraformaldehyde-fixed, paraffin-embedded human glioblastoma multiforme brain tissue stained for CD133 using ab19898 at 1/100 dilution in immunohistochemical analysis.

    See Abreview

  • Formaldehyde-fixed, 0.1% Triton-BSA permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells stained for CD133 (red) using ab19898 at 1/50 dilution in ICC/IF, followed by Goat Anti-Rabbit IgG (H+L) Cy3. Nuclei staining with Hoechst (blue).

    See Abreview

  • Formaldehyde-fixed, paraffin-embedded rat kidney tissue stained for CD133 using ab19898 at 1/1500 dilution in immunohistochemical analysis, followed by Goat Anti Rabbit IgG Biotin.

    See Abreview

References

This product has been referenced in:
  • Zhai R  et al. Pharmacological Mobilization of Endogenous Bone Marrow Stem Cells Promotes Liver Regeneration after Extensive Liver Resection in Rats. Sci Rep 8:3587 (2018). Read more (PubMed: 29483616) »
  • Zhao C  et al. ETV2 mediates endothelial transdifferentiation of glioblastoma. Signal Transduct Target Ther 3:4 (2018). Read more (PubMed: 29527330) »
See all 63 Publications for this product

Customer reviews and Q&As

1-10 of 48 Abreviews or Q&A

Question
Answer

Thank you for contacting Abcam regarding ab19898.


Attached please find a copy of the datasheet you requested.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions or concerns.

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Question
Answer

Thank you for contacting us.

I regret to inform you that the patent issues for this antibody still hasn't been cleared so we are unable to offer this antibody. We acknowledge that this might affect your research work however as we are bound by the patent legislation so we are unable to help in this case.

We also can not provide any date by which the product will be available.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of alternative antibody ab41834 with the order number ####.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question
Answer

Thank you for contacting us.

I am sorry to say we unfortunately would not be able to release this product unless the intellectual property issue has been resolved which is still ongoing. We also cannot comment on the republishing dates. I would suggest searching a similar product in Biocompare website at www.biocompare.com

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting us.

This product was unpublished due to patent issues. We are unfortunately not allowed to publish this product. I would suggest searching the product in biocompare website at http://www.biocompare.com/.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your reply.

We produced the antibody, ab19898 in house, but we licensed the production of it from original supplier. Unfortunately I do not have access to the information about who originally supplied, held the license for this antibody.

I am sorry that I could not be more help in this matter.

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Answer

Thank you for contacting Abcam.

Unfortunately I do not have the information about who we originally licensed this product from, however if you search http://www.biocompare.com/, I am sure you will find the same antibody there.

If there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting Abcam regarding ab19898.

Unfortunately, due to licensing issues we are no longer able to sell this product. If you do publish data using this antibody, you can list it as you have done.

I hope this information is helpful and I apologize for any inconvenience this may cause. Please do not hesitate to contact us if you have any additional questions or concerns.

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Answer

Vielen Dank für Ihre Anfrage. Es tut mir leid zu hören, dass dieses Batch nicht funktioniert. Ich weiß, dass Sie viel Zeit für diese Experimente verwendet haben und möchte Ihnen daher eine Gutschrift oder einen Gutschein als Ersatz anbieten (vorausgesetzt, das Produkt wurde in den letzten sechs Monaten gekauft). Dafür benötige ich jedoch ihre ursprüngliche BesteVielen Dank für Ihre Anfrage. Es tut mir leid zu hören, dass dieses Batch nicht funktioniert. Ich weiß, dass Sie viel Zeit für diese Experimente verwendet haben und möchte Ihnen daher eine Gutschrift oder einen Gutschein als Ersatz anbieten (vorausgesetzt, das Produkt wurde in den letzten sechs Monaten gekauft). Dafür benötige ich jedoch ihre ursprüngliche Bestellnummer oder einen Kontakt in Ihrer Einkaufsabteilung und das Datum der Bestellung. Aus lizenzrechtlichen Gründen, auf die wir leider keinen Einfluss haben, können wir im Moment keine Antikörper gegen CD133 anbieten. Uns ist bewusst, dass dieser Umstand für unsere Kunden zu Schwierigkeiten führen kann und möchten uns dafür entschuldigen. Vielen Dank für Ihre Kooperation und Ihr Verstaendnis. Ich freue mich bald wieder von Ihnen zu hören. lnummer oder einen Kontakt in Ihrer Einkaufsabteilung und das Datum der Bestellung. Aus lizenzrechtlichen Gründen, auf die wir leider keinen Einfluss haben, können wir im Moment keine Antikörper gegen CD133 anbieten. Uns ist bewusst, dass dieser Umstand für unsere Kunden zu Schwierigkeiten führen kann und möchten uns dafür entschuldigen.   Vielen Dank für Ihre Kooperation und Ihr Verstaendnis. Ich freue mich bald wieder von Ihnen zu hören.    

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1-10 of 48 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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