Recombinant
RabMAb

Recombinant Anti-CD133 antibody [EPR20980-45] (ab226355)

Overview

  • Product name

    Anti-CD133 antibody [EPR20980-45]
    See all CD133 primary antibodies
  • Description

    Rabbit monoclonal [EPR20980-45] to CD133
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD133 aa 500-800. The exact sequence is proprietary.
    Database link: O43490

  • Positive control

    • WB: Caco-2, NT2/D1 and HT-29 whole cell lysate. Human pancreas lysate and fetal kidney lysate. IHC-P: Human pancreas tissue. Human pancreatic cancer tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 97 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
  • Tissue specificity

    Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).
  • Involvement in disease

    Retinitis pigmentosa 41
    Cone-rod dystrophy 12
    Stargardt disease 4
    Retinal macular dystrophy 2
  • Sequence similarities

    Belongs to the prominin family.
  • Post-translational
    modifications

    Isoform 1 and isoform 2 are glycosylated.
    Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
  • Cellular localization

    Apical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.
  • Information by UniProt
  • Database links

  • Alternative names

    • AC133 antibody
    • Antigen AC133 antibody
    • CD133 antibody
    • CORD12 antibody
    • Hematopoietic stem cell antigen antibody
    • hProminin antibody
    • MCDR2 antibody
    • MSTP061 antibody
    • OTTHUMP00000217744 antibody
    • OTTHUMP00000217745 antibody
    • OTTHUMP00000217746 antibody
    • PROM1 antibody
    • PROM1_HUMAN antibody
    • Prominin I antibody
    • Prominin like 1 antibody
    • Prominin like protein 1 precursor antibody
    • Prominin mouse like 1 antibody
    • Prominin-1 antibody
    • Prominin-like protein 1 antibody
    • Prominin1 antibody
    • PROML1 antibody
    • RP41 antibody
    • STGD4 antibody
    see all

Images

  • All lanes : Anti-CD133 antibody [EPR20980-45] (ab226355) at 1/1000 dilution

    Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : NCCIT (human pluripotent embryonic carcinoma epithelial cell), whole cell lysate
    Lane 4 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 97 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking: 5% NFDM/TBST.

    The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).

    Negative control: HEK-293T (PMID 20167130).

  • All lanes : Anti-CD133 antibody [EPR20980-45] (ab226355) at 1/1000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : NT2/D1 (human malignant pluripotent embryonic carcinoma epithelial cell-like), whole cell lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 97 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking: 5% NFDM/TBST.

    Exposure times:

    Lane 1: 3 minutes

    Lanes 2-3: 10 seconds

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD133 with ab226355 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Apical/endoluminal staining on ducts of human pancreas, and negative on Langerhans cells of the islets (PMID: 18261235) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling CD133 with ab226355 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Apical/endoluminal staining on ducts of human pancreatic cancer (PMID: 18261235) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

References

ab226355 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Nov 30 2017

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