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    cd133-antibody-mabcam27699-ab27699.pdf

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Neuroscience Cell Type Marker Neural Stem Cell marker
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Anti-CD133 antibody [mAbcam27699] (ab27699)

  • Datasheet
Reviews (6)Q&A (2)References (9)

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Western blot - Anti-CD133 antibody [mAbcam27699] (ab27699)

    Key features and details

    • Mouse monoclonal [mAbcam27699] to CD133
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG2b

    You may also be interested in

    Primary
    Product image
    Anti-CD44 antibody (ab157107)
    Protein
    Product image
    Recombinant Human CD133 protein (ab160218)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-CD133 antibody [mAbcam27699]
      See all CD133 primary antibodies
    • Description

      Mouse monoclonal [mAbcam27699] to CD133
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse CD133.

      Read Abcam's proprietary immunogen policy (Peptide available as ab31714.)
    • Positive control

      • WB: Mouse kidney tissue lysate.
    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      mAbcam27699
    • Myeloma

      Sp2
    • Isotype

      IgG2b
    • Light chain type

      kappa
    • Research areas

      • Neuroscience
      • Cell Type Marker
      • Neural Stem Cell marker
      • Stem Cells
      • Neural Stem Cells
      • Surface Molecules
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Neuroscience
      • Development

    Associated products

    • Isotype control

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
    • Recombinant Protein

      • Recombinant Human CD133 protein (ab160218)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab27699 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
    Notes
    WB
    Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).

    Target

    • Function

      May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
    • Tissue specificity

      Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).
    • Involvement in disease

      Retinitis pigmentosa 41
      Cone-rod dystrophy 12
      Stargardt disease 4
      Retinal macular dystrophy 2
    • Sequence similarities

      Belongs to the prominin family.
    • Post-translational
      modifications

      Isoform 1 and isoform 2 are glycosylated.
      Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
    • Cellular localization

      Apical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.
    • Target information above from: UniProt accession O43490 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 19126 Mouse
      • SwissProt: O54990 Mouse
      • Unigene: 6250 Mouse
      • Alternative names

        • AC133 antibody
        • Antigen AC133 antibody
        • CD133 antibody
        • CORD12 antibody
        • Hematopoietic stem cell antigen antibody
        • hProminin antibody
        • MCDR2 antibody
        • MSTP061 antibody
        • OTTHUMP00000217744 antibody
        • OTTHUMP00000217745 antibody
        • OTTHUMP00000217746 antibody
        • PROM1 antibody
        • PROM1_HUMAN antibody
        • Prominin I antibody
        • Prominin like 1 antibody
        • Prominin like protein 1 precursor antibody
        • Prominin mouse like 1 antibody
        • Prominin-1 antibody
        • Prominin-like protein 1 antibody
        • Prominin1 antibody
        • PROML1 antibody
        • RP41 antibody
        • STGD4 antibody
        see all

      Images

      • Western blot - Anti-CD133 antibody [mAbcam27699] (ab27699)
        Western blot - Anti-CD133 antibody [mAbcam27699] (ab27699)
        Anti-CD133 antibody [mAbcam27699] (ab27699) at 5 µg + Mouse kidney tissue lysate at 20 µg

        Secondary
        HRP conjugated goat anti-mouse at 1/5000 dilution

        Predicted band size: 97 kDa
        Observed band size: 120 kDa why is the actual band size different from the predicted?
        Additional bands at: 55 kDa (possible IgG)



        This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab27699 overnight at 4°C. Antibody binding was detected using a goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (9)

      Publishing research using ab27699? Please let us know so that we can cite the reference in this datasheet.

      ab27699 has been referenced in 9 publications.

      • Nosrati A  et al. Cancer Stem Cell Markers CD44, CD133 in Primary Gastric Adenocarcinoma. Int J Mol Cell Med 3:279-86 (2014). IHC-P ; Human . PubMed: 25635255
      • Lubanska D  et al. The cyclin-like protein Spy1 regulates growth and division characteristics of the CD133+ population in human glioma. Cancer Cell 25:64-76 (2014). ICC/IF ; Human . PubMed: 24434210
      • Ou J  et al. Fibronectin extra domain A (EDA) sustains CD133(+)/CD44(+) subpopulation of colorectal cancer cells. Stem Cell Res 11:820-33 (2013). PubMed: 23811539
      • Grosse-Gehling P  et al. CD133 as a biomarker for putative cancer stem cells in solid tumours: limitations, problems and challenges. J Pathol 229:355-78 (2013). PubMed: 22899341
      • De Las Heras M  et al. Solitary Tumours Associated with Jaagsiekte Retrovirus in Sheep are Heterogeneous and Contain Cells Expressing Markers Identifying Progenitor Cells in Lung Repair. J Comp Pathol : (2013). IHC ; Human . PubMed: 24176105
      • Feldman DE  et al. Pluripotency factor-mediated expression of the leptin receptor (OB-R) links obesity to oncogenesis through tumor-initiating stem cells. Proc Natl Acad Sci U S A 109:829-34 (2012). PubMed: 22207628
      • Blackmore DG  et al. Growth hormone responsive neural precursor cells reside within the adult mammalian brain. Sci Rep 2:250 (2012). PubMed: 22355762
      • Dubrovska A  et al. CXCR4 expression in prostate cancer progenitor cells. PLoS One 7:e31226 (2012). PubMed: 22359577
      • Becker L  et al. Immunostaining of Lgr5, an intestinal stem cell marker, in normal and premalignant human gastrointestinal tissue. ScientificWorldJournal 8:1168-76 (2008). IHC-P ; Human . PubMed: 19030762

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-8 of 8 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD133 antibody [mAbcam27699]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Mouse day 5 colon)
      Specification
      Mouse day 5 colon
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Apr 07 2009

      Flow Cytometry abreview for Anti-CD133 antibody [mAbcam27699]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (KM-H2)
      Specification
      KM-H2
      Preparation
      Cell harvesting/tissue preparation method: Asynchronous KM-H2 were harvested and labeled by indirect immunofluorescence
      Sample buffer: PBS + 5% FBS
      Permeabilization
      No
      Gating Strategy
      Forward/Side scatter were used to eliminate cellular debris. The accompanying marker was applied such that only 2% of the IgG control was positive.
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Kirk Mcmanus

      Verified customer

      Submitted Dec 16 2008

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD133 antibody [mAbcam27699]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (jejunum)
      Specification
      jejunum
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid pH6
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Carl Hobbs

      Verified customer

      Submitted May 07 2008

      Western blot abreview for Anti-CD133 antibody [mAbcam27699]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Multiple cell lines, including U2OS)
      Loading amount
      25 µg
      Specification
      Multiple cell lines, including U2OS
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Feb 25 2008

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD133 antibody [mAbcam27699]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Kidney)
      Specification
      Kidney
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 14 2007

      Immunocytochemistry/ Immunofluorescence abreview for Anti-CD133 antibody [mAbcam27699]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Renal (proximal tuular) epithelial cell line)
      Specification
      Renal (proximal tuular) epithelial cell line
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.5% Tritonx-100 (PBS)
      Blocking step
      BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 14 2007

      Question

      Inquiry: rabbit derived anti cd133 antibody against mouse for IHC & WB available?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 16 2012

      Answer

      Thank you for your inquiry.

      Unfortunately, we do not have any antibodies againstCD133 available in our catalog.
      I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:
      www.biocompare.com
      http://www.biocompare.com/ProductCategories/2045/Antibodies.html

      We are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services visit:

      http://www.epitomics.com/services/
      email: service@epitomics.com

      Alternatively, please contact Li Li at Epitomics:

      li.li@epitomics.com

      Read More

      Abcam Scientific Support

      Answered on Oct 16 2012

      Question

      Dear Abcam:

      One of our customers(Miss Du) bought an antibody of yours when she was in USA, product code number: ab27699. Unfortunately your are no longer able to supply this product. Now she is using this antibody in her research, but she lost the data sheet. Can you tell me the concentration in FC application?

      Thank you!

      Best Regards!





      Shen Wang︱Product Support Specialist
      Amyjet Scientific Inc.
      Wuhan, Hubei, China 430074
      Office: (0086)027-84454412-808 Fax: (0086)027-84454876
      Mail: mailto:shen.wang@amyjet.com Web: http://www.amyjet.com/

      Read More

      Abcam community

      Verified customer

      Asked on May 31 2012

      Answer

      Thank you for contacting us.

      Does FC mean flow cytometry? According to our records, ab27699 was tested for WB and IHC-P only.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on May 31 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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