Overview

  • Product name

    Anti-CD133 antibody - Stem Cell Marker
    See all CD133 primary antibodies
  • Description

    Rabbit polyclonal to CD133 - Stem Cell Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, WB, ELISA, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    KDHVYGIHNPVMTSPSQH

    , corresponding to C terminal amino acids 848-865 of CD133.

  • Positive control

    • WB: Y79 cell lysate. ICC: cultured primary human kidney cells IHC: Hepatocarcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab16518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
ICC/IF 1/10 - 1/50.

Fix with 3% paraformaldehyde for 20 min at RT.

Flow Cyt 1/10 - 1/50.
IHC-P 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
ELISA Use at an assay dependent concentration.
IHC-FoFr 1/100. PubMed: 17320377

Target

  • Function

    May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
  • Tissue specificity

    Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).
  • Involvement in disease

    Retinitis pigmentosa 41
    Cone-rod dystrophy 12
    Stargardt disease 4
    Retinal macular dystrophy 2
  • Sequence similarities

    Belongs to the prominin family.
  • Post-translational
    modifications

    Isoform 1 and isoform 2 are glycosylated.
    Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
  • Cellular localization

    Apical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.
  • Information by UniProt
  • Database links

  • Alternative names

    • AC133 antibody
    • Antigen AC133 antibody
    • CD133 antibody
    • CORD12 antibody
    • Hematopoietic stem cell antigen antibody
    • hProminin antibody
    • MCDR2 antibody
    • MSTP061 antibody
    • OTTHUMP00000217744 antibody
    • OTTHUMP00000217745 antibody
    • OTTHUMP00000217746 antibody
    • PROM1 antibody
    • PROM1_HUMAN antibody
    • Prominin I antibody
    • Prominin like 1 antibody
    • Prominin like protein 1 precursor antibody
    • Prominin mouse like 1 antibody
    • Prominin-1 antibody
    • Prominin-like protein 1 antibody
    • Prominin1 antibody
    • PROML1 antibody
    • RP41 antibody
    • STGD4 antibody
    see all

Images

  • ab16518 staining CD133 in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). ab16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
  • ab16518 recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation. ab16518 recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.
  • IF staining for nestin and CD133 using CD133 antibody (ab16518) in primary human kidney cells isolated from the loop of Henle of human kidney papilla. The cells were fixed with 3% paraformaldehyde for 20 min at room temperature. CD133 is red (c), nestin is green (b) and blue channel is beta-catenin used as a cell membrane outline (a). Image d shows the merged image of all three. CD133 antibody (ab16518) at a dilution of 1:100.

  • IF staining of CD133 in cultured primary human kidney cells isolated from the human papilla. The cells were fixed with 3% paraformaldehyde for 20 min at RT. CD133 antibody (ab16518) was used at dilution of 1:100.
  • Anti-CD133 antibody - Stem Cell Marker (ab16518) at 1 µg/ml + mouse embryonic stem cell lysate at 10 µg

    Secondary
    Goat anti-rabbit at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 120,130 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

  • Paraformaldehyde-fixed mouse mammary carcinoma tissue stained for CD133 (red) using ab16518 at 1/100 dilution in immunohistochemical analysis.

    See Abreview

  • ab16518 staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence
  • ab16518 staining CD133 in Mouse pancreatic neoplasia tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone/methanol (1:1) and blocked with 10% serum for 1 hour. Samples were incubated with primary antibody (1/100) for 8 hours at 4°C. An Alexa Fluor®555-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
    CD133 staining in red; b-integrin staining in green and DAPI staining in blue

    See Abreview

  • Flow cytometric analysis of CEM cells using ab16518 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:

  • Wu XB  et al. Cross-talk among AFAP1-AS1, ACVR1 and microRNA-384 regulates the stemness of pancreatic cancer cells and tumorigenicity in nude mice. J Exp Clin Cancer Res 38:107 (2019). Read more (PubMed: 30819221) »
  • Yin L  et al. Sulforaphane Induces miR135b-5p and Its Target Gene, RASAL2, thereby Inhibiting the Progression of Pancreatic Cancer. Mol Ther Oncolytics 14:74-81 (2019). Read more (PubMed: 31044154) »
See all 32 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (neurospheres)
Permeabilization
No
Specification
neurospheres
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 14 2018

Answer

Vielen Dank für Ihre Anfrage.

Das Datenblatt finden Sie im Anhang, leider etwas "zerstückelt", da unser System keine PDF von einem unveröffentlichten Antikörper herstellen kann.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Answer

Vielen Dank für Ihre Anfrage.

Leider können wir aus lizenzrechtlichen Gründen keinerlei anti CD133 Antikörper mehr anbieten.

Wir haben unser möglichstes getan eine Lösung zu finden und die sehr populären Antikörper weiterhin anbieten zu können, sind aber nicht erfolgreich gewesen.

Wir entschuldigen uns vielmals die dadurch verursachten Umstände.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (pancreatic cancer)
Loading amount
30 µg
Specification
pancreatic cancer
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 22 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse pancreatic neoplasia (PanINs))
Specification
mouse pancreatic neoplasia (PanINs)
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 12 2010

Application
Western blot
Sample
Mouse Cell lysate - whole cell (pancreatic cancer)
Loading amount
35 µg
Specification
pancreatic cancer
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Dr. Pawel Mazur

Verified customer

Submitted Jun 16 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (pancreatic tumor)
Specification
pancreatic tumor
Fixative
Acetone/Methanol 1:1
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Dr. Pawel Mazur

Verified customer

Submitted May 28 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (glioma specimen)
Specification
glioma specimen
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 30 2009

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (mammary carcinoma)
Specification
mammary carcinoma
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 28 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Glioblastoma-Brain tumor)
Specification
Glioblastoma-Brain tumor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Avidin biotin/ Fc receptor and Background Buster as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: predilute

Abcam user community

Verified customer

Submitted May 09 2007

1-10 of 15 Abreviews or Q&A

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