Overview

  • Product name

    Anti-CD134 / OX40L receptor antibody [OX40]
    See all CD134 / OX40L receptor primary antibodies
  • Description

    Mouse monoclonal [OX40] to CD134 / OX40L receptor
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to CD134/ OX40L receptor. PHA-activated rat lymph node cells.

  • Positive control

    • IHC-Fr: Rat spleen tissue. FC: Lewis rat splenocytes treated with 5 µg/ml ConA for 3 days.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab238475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use a concentration of 5 µg/ml.

Target

  • Function

    Receptor for TNFSF4/OX40L/GP34.
  • Sequence similarities

    Contains 4 TNFR-Cys repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACT 35 antibody
    • ACT35 antibody
    • ACT35 antigen antibody
    • ATC35 antigen antibody
    • CD 134 antibody
    • CD134 antibody
    • CD134 antigen antibody
    • IMD16 antibody
    • Lymphoid activation antigene ACT35 antibody
    • OX 40 antibody
    • OX40 antibody
    • OX40 antigen antibody
    • OX40 cell surface antigen antibody
    • OX40 homologue antibody
    • OX40L receptor antibody
    • TAX transcriptionally activated glycoprotein 1 receptor antibody
    • TAX transcriptionally-activated glycoprotein 1 receptor antibody
    • TNF receptor superfamily member 4 antibody
    • TNFRSF 4 antibody
    • TNFRSF4 antibody
    • TNR4_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 4 antibody
    • Txgp 1l antibody
    • Txgp1 antibody
    • Txgp1l antibody
    see all

Images

  • Lewis rat splenocytes (top) or Lewis rat splenocytes treated with 5µg/ml ConA for 3 days (bottom) were stained with ab238475 (right) or mouse IgG2b kappa (ab170192) isotype (left). Splenocytes were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab238475) or mouse IgG2b kappa (ab170192) isotype (1x 106 in 100µl at 0.2µg/ml) for 30 min on ice.

    The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor®488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD4.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on CD3 positive T cells.

  • IHC image of CD134/OX40L receptor staining in a section of frozen normal rat spleen*.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab238475 at 5µg/ml. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647, 1/1000)) (shown in red) for 1 hour at room temperature. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

    *Tissue obtained from Charles River.

References

ab238475 has not yet been referenced specifically in any publications.

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