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    cd134--ox40l-receptor-antibody-sp195-c-terminal-ab228464.pdf

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Immunology Adaptive Immunity B Cells CD
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RecombinantRabMAb

Recombinant Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)
  • Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP195] to CD134 / OX40L receptor - C-terminal
  • Suitable for: IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)

View more associated products

Overview

  • Product name

    Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal
    See all CD134 / OX40L receptor primary antibodies
  • Description

    Rabbit monoclonal [SP195] to CD134 / OX40L receptor - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue.
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP195
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free (ab245756)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab228464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

Notes
IHC-P
1/100.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    Receptor for TNFSF4/OX40L/GP34.
  • Sequence similarities

    Contains 4 TNFR-Cys repeats.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P43489 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7293 Human
    • Omim: 600315 Human
    • SwissProt: P43489 Human
    • Unigene: 129780 Human
    • Alternative names

      • ACT 35 antibody
      • ACT35 antibody
      • ACT35 antigen antibody
      • ATC35 antigen antibody
      • CD 134 antibody
      • CD134 antibody
      • CD134 antigen antibody
      • IMD16 antibody
      • Lymphoid activation antigene ACT35 antibody
      • OX 40 antibody
      • OX40 antibody
      • OX40 antigen antibody
      • OX40 cell surface antigen antibody
      • OX40 homologue antibody
      • OX40L receptor antibody
      • TAX transcriptionally activated glycoprotein 1 receptor antibody
      • TAX transcriptionally-activated glycoprotein 1 receptor antibody
      • TNF receptor superfamily member 4 antibody
      • TNFRSF 4 antibody
      • TNFRSF4 antibody
      • TNR4_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 4 antibody
      • Txgp 1l antibody
      • Txgp1 antibody
      • Txgp1l antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD134 / OX40L receptor with ab228464 at 1/100 dilution (4.63 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD134 / OX40 using ab228464 at 1/100 dilution in immunohistochemical analysis.

    • Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)
      Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab228464? Please let us know so that we can cite the reference in this datasheet.

    ab228464 has not yet been referenced specifically in any publications.

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