Recombinant Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP195] to CD134 / OX40L receptor - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal
See all CD134 / OX40L receptor primary antibodies -
Description
Rabbit monoclonal [SP195] to CD134 / OX40L receptor - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD134/ OX40L receptor aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P43489 -
Positive control
- IHC-P: Human tonsil tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP195 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab228464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature. |
Target
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Function
Receptor for TNFSF4/OX40L/GP34. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7293 Human
- Omim: 600315 Human
- SwissProt: P43489 Human
- Unigene: 129780 Human
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Alternative names
- ACT 35 antibody
- ACT35 antibody
- ACT35 antigen antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)](https://www.abcam.com/ps/products/228/ab228464/Images/ab228464-2-AntiCD134--OX40L-receptor-antibody-SP195--Cterminal-IHC-Human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD134 / OX40L receptor with ab228464 at 1/100 dilution (4.63 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)](https://www.abcam.com/ps/products/228/ab228464/Images/ab228464-293305-anti-cd134-ox40l-receptor-antibody-sp195-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - C-terminal (ab228464)Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD134 / OX40 using ab228464 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab228464 has not yet been referenced specifically in any publications.
