Recombinant Anti-CD137 antibody [BLR051F] - BSA free (ab252559)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR051F] to CD137 - BSA free
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-CD137 antibody [BLR051F] - BSA free
See all CD137 primary antibodies -
Description
Rabbit monoclonal [BLR051F] to CD137 - BSA free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD137. Extracellular domain.
Database link: Q07011 -
Positive control
- IHC-P: Human tonsil tissue. ICC: HCLM2 cells. WB: HDLM whole cell lysate. IP: HDLM2 whole cell lysate.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR051F -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 28 kDa.
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IP |
Use at an assay dependent concentration.
6 µl/1 mg lysate |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. |
Notes |
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WB
1/1000. Predicted molecular weight: 28 kDa. |
IP
Use at an assay dependent concentration. 6 µl/1 mg lysate |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. |
Target
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Function
Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation. -
Tissue specificity
Expressed on the surface of activated T-cells. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3604 Human
- Omim: 602250 Human
- SwissProt: Q07011 Human
- Unigene: 654459 Human
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Alternative names
- 4 1BB antibody
- 4 1BB ligand receptor antibody
- 4-1BB ligand receptor antibody
see all
Images
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD37 with ab252559 at a 1/200 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
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All lanes : Anti-CD137 antibody [BLR051F] - BSA free (ab252559) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : SUP-M2 (Human cell line) whole cell lysate
Lane 5 : HDML2 (Human cell line) whole cell lysate
Lane 6 : My-La-CD4+ (Human cell line) whole cell lysate
Lane 7 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 28 kDa
Exposure time: 3 minutesPrepared using NETN lysis buffer.
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CD37 was immunoprecipitated from HDLM2 (Human cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252559 used for IP at 6 µl per reaction. For WB 1/1000 dilution.Lane 1: ab252559 IP in HDLM2 whole cell lysate.
Lane 2: Control IgG IP in HDLM2 whole cell lysate.
Chemiluminescence detection: 30 seconds. -
FFPE section of HDLM2 (Human cell line) cells labeling CD37 using ab252559 at a 1/200 dilution in ICC.
Secondary: HRP-conjugated goat anti-rabbit IgG.
Substrate: DAB.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab252559 has not yet been referenced specifically in any publications.