Recombinant Anti-CD137 antibody [EPR20238] (ab209256)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20238] to CD137
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD137 antibody [EPR20238]
See all CD137 primary antibodies -
Description
Rabbit monoclonal [EPR20238] to CD137 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: His-tagged CD137 Fc chimera recombinant protein containing human CD137 (aa24-186); Human lymphoma and fetal spleen lysates; H9 whole cell lysate; whole cell lysate from Jurkat cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (TPA) and 1µM Ionomycin (ab120116) for 48 hours.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20238 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 36, 40 kDa (predicted molecular weight: 28 kDa).
|
Notes |
---|
WB
1/1000. Detects a band of approximately 36, 40 kDa (predicted molecular weight: 28 kDa). |
Target
-
Function
Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation. -
Tissue specificity
Expressed on the surface of activated T-cells. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3604 Human
- Omim: 602250 Human
- SwissProt: Q07011 Human
- Unigene: 654459 Human
-
Alternative names
- 4 1BB antibody
- 4 1BB ligand receptor antibody
- 4-1BB ligand receptor antibody
see all
Images
-
Anti-CD137 antibody [EPR20238] (ab209256) at 1/5000 dilution + His-tagged CD137 Fc chimera recombinant protein containing human CD137 (aa24-186) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 4 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution
Lane 1 : Human lymphoma lysate
Lane 2 : Human fetal spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The MW observed is consistent with the literature: PMID 10528184; PMID 17560335.
-
Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution + H9 (Human cutaneous T Lymphocyte cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 36,40 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The MW observed is consistent with the literature: PMID 10528184; PMID 17560335.
-
All lanes : Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution
Lane 1 : Whole cell lysate from untreated Jurkat (Human T cell leukemia cell line from peripheral blood)
Lane 2 : Whole cell lysate from Jurkat cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (TPA) and 1µM Ionomycin (ab120116) for 48 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 36,40 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression level of CD137 can be induced by TPA and Ionomycin [PMID 14648705].
The MW observed is consistent with the literature: PMID 10528184; PMID 17560335.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab209256 has not yet been referenced specifically in any publications.