Overview

  • Product name

    Anti-CD137 antibody [EPR20238]
    See all CD137 primary antibodies
  • Description

    Rabbit monoclonal [EPR20238] to CD137
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD137 aa 1-200. The exact sequence is proprietary.
    Database link: Q07011

  • Positive control

    • WB: His-tagged CD137 Fc chimera recombinant protein containing human CD137 (aa24-186); Human lymphoma and fetal spleen lysates; H9 whole cell lysate; whole cell lysate from Jurkat cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (TPA) and 1µM Ionomycin (ab120116) for 48 hours.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 36, 40 kDa (predicted molecular weight: 28 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation.
    • Tissue specificity

      Expressed on the surface of activated T-cells.
    • Sequence similarities

      Contains 4 TNFR-Cys repeats.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 4 1BB antibody
      • 4 1BB ligand receptor antibody
      • 4-1BB ligand receptor antibody
      • 4-1BB Ligand Receptor T Cell antibody
      • 4-1BB, mouse, homolog of antibody
      • Antigen 4-1BB Homolog antibody
      • CD 137 antibody
      • CD137 antibody
      • CD137 antigen antibody
      • CDw137 antibody
      • HLDA VI antibody
      • Homolog of mouse 4 1BB antibody
      • ILA antibody
      • induced by lymphocyte activation (ILA) antibody
      • Induced by lymphocyte activation antibody
      • Interleukin activated receptor homolog of mouse Ly63 antibody
      • Ly63, mouse, homolog of antibody
      • MGC2172 antibody
      • OTTHUMP00000044294 antibody
      • Receptor protein 4 1BB antibody
      • T cell antigen 4 1BB homolog antibody
      • T cell antigen ILA antibody
      • T-cell antigen 4-1BB homolog antibody
      • T-cell antigen ILA antibody
      • TNF receptor superfamily member 9 antibody
      • TNFRSF9 antibody
      • TNR9_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 9 antibody
      see all

    Images

    • Anti-CD137 antibody [EPR20238] (ab209256) at 1/5000 dilution + His-tagged CD137 Fc chimera recombinant protein containing human CD137 (aa24-186) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 28 kDa
      Observed band size: 65 kDa
      why is the actual band size different from the predicted?


      Exposure time: 4 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution

      Lane 1 : Human lymphoma lysate
      Lane 2 : Human fetal spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 28 kDa
      Observed band size: 36 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The MW observed is consistent with the literature: PMID 10528184; PMID 17560335.

    • Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution + H9 (Human cutaneous T Lymphocyte cell line) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 28 kDa
      Observed band size: 36,40 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The MW observed is consistent with the literature: PMID 10528184; PMID 17560335.

    • All lanes : Anti-CD137 antibody [EPR20238] (ab209256) at 1/1000 dilution

      Lane 1 : Whole cell lysate from untreated Jurkat (Human T cell leukemia cell line from peripheral blood)
      Lane 2 : Whole cell lysate from Jurkat cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (TPA) and 1µM Ionomycin (ab120116) for 48 hours

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 28 kDa
      Observed band size: 36,40 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The expression level of CD137 can be induced by TPA and Ionomycin [PMID 14648705].

      The MW observed is consistent with the literature: PMID 10528184; PMID 17560335.

    References

    ab209256 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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