Recombinant Anti-CD137 antibody [EPR25096-57] (ab300349)
![Western blot - Anti-CD137 antibody [EPR25096-57] (AB300349)](https://www.abcam.com/ps/products/300/ab300349/Images/AB300349-486625-anti-tumor-necrosis-factor-receptor-superfamily-member-9-epr-western-blot.jpg "Western blot - Anti-CD137 antibody [EPR25096-57] (AB300349)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25096-57] to CD137
- Suitable for: Flow Cyt, IP, WB, ICC
- Reacts with: Human
Overview
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Product name
Anti-CD137 antibody [EPR25096-57]
See all CD137 primary antibodies -
Description
Rabbit monoclonal [EPR25096-57] to CD137 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IP, WB, ICCmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HDLM-2 (human Hodgkin lymphoma) whole cell lysate, Human tonsil tissue lysate, Human lymphoma tissue lysate. ICC: HDML-2 cell line. Flow cyt.: HDML-2 cell line. IP: HDML-2 cell line.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25096-57 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/500.
|
|
| IP |
1/30.
|
|
| WB |
1/1000. Detects a band of approximately 32, 40 kDa (predicted molecular weight: 28 kDa).
|
|
| ICC |
1/500.
|
| Notes |
|---|
|
Flow Cyt
1/500. |
|
IP
1/30. |
|
WB
1/1000. Detects a band of approximately 32, 40 kDa (predicted molecular weight: 28 kDa). |
|
ICC
1/500. |
Target
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Function
Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation. -
Tissue specificity
Expressed on the surface of activated T-cells. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3604 Human
- Omim: 602250 Human
- SwissProt: Q07011 Human
- Unigene: 654459 Human
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Alternative names
- 4 1BB antibody
- 4 1BB ligand receptor antibody
- 4-1BB ligand receptor antibody
see all
Images
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Western blot - Anti-CD137 antibody [EPR25096-57] (AB300349)All lanes : Anti-CD137 antibody [EPR25096-57] (ab300349) at 1/1000 dilution
Lane 1 : HDLM-2 (human Hodgkin lymphoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lysates/proteins at 1/20 dilution per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 32, 40 kDa why is the actual band size different from the predicted?
Exposure time: 103 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 103 seconds.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 30355497; PMID: 31244935).
Negative control: Jurkat (PMID: 14648705; PMID: 25409517). -
![Western blot - Anti-CD137 antibody [EPR25096-57] (AB300349)](https://www.abcam.com/ps/products/300/ab300349/Images/AB300349-486627-anti-tumor-necrosis-factor-receptor-superfamily-member-9-epr2509657-western-blot2.jpg)
Western blot - Anti-CD137 antibody [EPR25096-57] (AB300349)All lanes : Anti-CD137 antibody [EPR25096-57] (ab300349) at 1/1000 dilution
Lane 1 : Human tonsil tissue lysate
Lane 2 : Human spleen tissue lysate
Lane 3 : Human lymphoma tissue lysate
Lysates/proteins at 1/20 dilution per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 32, 40 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 30355497; PMID: 31244935).
Low expressed: human spleen (PMID: 22901750). -
![Immunocytochemistry - Anti-CD137 antibody [EPR25096-57] (AB300349)](https://www.abcam.com/ps/products/300/ab300349/Images/AB300349-486629-anti-tumor-necrosis-factor-receptor-superfamily-member-9-epr2509657-immunocytochemistry-hdml.jpg)
Immunocytochemistry - Anti-CD137 antibody [EPR25096-57] (AB300349)Immunofluorescent analysis of 80% methanol fixed and 0.1% TritonX-100 permeabilized HDLM-2 (human Hodgkin lymphoma) labeling CD137 with ab300349 at 1/500 dilution followed by preadsorbed Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488, Green, ab150081) at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in HDLM-2 cell line.
Negative control: Jurkat (PMID: 25409517).Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594, Red, ab195889) at 1/200 dilution was used as a counterstain. Nuclear counterstain is DAPI.
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![Flow Cytometry - Anti-CD137 antibody [EPR25096-57] (AB300349)](https://www.abcam.com/ps/products/300/ab300349/Images/AB300349-486631-anti-tumor-necrosis-factor-receptor-superfamily-member-9-epr2509657-flow-cyt-jurkat-hdml2.jpg)
Flow Cytometry - Anti-CD137 antibody [EPR25096-57] (AB300349)Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / HDLM-2 (Human Hodgkin lymphoma, Right) cells labeling CD137 with ab300349 at 1/500 dilution (Red) compared with isotype control Rabbit monoclonal IgG (ab172730) / Black and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as secondary antibody (Green).
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![Immunoprecipitation - Anti-CD137 antibody [EPR25096-57] (AB300349)](https://www.abcam.com/ps/products/300/ab300349/Images/AB300349-486633-anti-tumor-necrosis-factor-receptor-superfamily-member-9-epr2509657-immunoprecipitation-hdml2.jpg)
Immunoprecipitation - Anti-CD137 antibody [EPR25096-57] (AB300349)CD137 was immunoprecipitated from HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 μg with ab300349 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab300349 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) at 1/5000 dilution was used as a secondary antibody.
Lane 1 (Input): HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 μg
Lane 2 (+): ab300349 IP in HDLM-2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab300349 in HDLM-2 whole cell lysate
Exposure time: 15 seconds.
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![Anti-CD137 antibody [EPR25096-57] (AB300349)](https://www.abcam.com/ps/products/300/ab300349/Images/AB300349-486622-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD137 antibody [EPR25096-57] (AB300349)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300349 has not yet been referenced specifically in any publications.