Overview

  • Product name

    Anti-CD137 antibody [MM0071-9G43]
    See all CD137 primary antibodies
  • Description

    Mouse monoclonal [MM0071-9G43] to CD137
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to the extracellular domain of Human CD137

Properties

Applications

Our Abpromise guarantee covers the use of ab89085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.

Target

  • Function

    Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation.
  • Tissue specificity

    Expressed on the surface of activated T-cells.
  • Sequence similarities

    Contains 4 TNFR-Cys repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4 1BB antibody
    • 4 1BB ligand receptor antibody
    • 4-1BB ligand receptor antibody
    • 4-1BB Ligand Receptor T Cell antibody
    • 4-1BB, mouse, homolog of antibody
    • Antigen 4-1BB Homolog antibody
    • CD 137 antibody
    • CD137 antibody
    • CD137 antigen antibody
    • CDw137 antibody
    • HLDA VI antibody
    • Homolog of mouse 4 1BB antibody
    • ILA antibody
    • induced by lymphocyte activation (ILA) antibody
    • Induced by lymphocyte activation antibody
    • Interleukin activated receptor homolog of mouse Ly63 antibody
    • Ly63, mouse, homolog of antibody
    • MGC2172 antibody
    • OTTHUMP00000044294 antibody
    • Receptor protein 4 1BB antibody
    • T cell antigen 4 1BB homolog antibody
    • T cell antigen ILA antibody
    • T-cell antigen 4-1BB homolog antibody
    • T-cell antigen ILA antibody
    • TNF receptor superfamily member 9 antibody
    • TNFRSF9 antibody
    • TNR9_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 9 antibody
    see all

References

ab89085 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry that a technical difficulty has delayed your receiving this replacement. I am also sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Answer

Thank you for contacting Abcam.

I am sorry to hear of the difficulties that you have been experiencing using these products. Would you mind sending me the details of your protocol with your sample preparations including denaturing and reducing steps, the amount of total protein per well. Also important are the controls you may have used, especially loading controls or poncheau staining results. Please include blocking procedures, antibody incubation steps, washes, images and any steps that you may have changes while troubleshooting.

These details will not only help me to better understand this issue and be able to offer suggestions but will also be used for internal testing of the products if deemed necessary. If we are unable to solve the problems you are experiencing we will replace or refund the antibody according to our Abpromise guarantee.

Read More

Answer

Thank you for contacting us. The immunogen is between aa to aa of human protein. This antibody should pick up the target protein at 30-35kDa. As the target protein could form dimers, is the 70kDa band its dimer molecules? We recommend using beta-Mercaptoethanol or DTT with anoinic denaturing detergent in your loading buffer and heating your sample at 95C in this reducing-denaturing buffer for 5 minutes. You may increase boiling time to 10 minutes if neccessary. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up