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The unspecific band is in our untransfected HEK293 cell supernatant. No band should show up in this supernatant.
Asked on Jan 27 2012
Thank you for contacting Abcam.
I am sorry to hear of the difficulties that you have been experiencing using these products. Would you mind sending me the details of your protocol with your sample preparations including denaturing and reducing steps, the amount of total protein per well. Also important are the controls you may have used, especially loading controls or poncheau staining results. Please include blocking procedures, antibody incubation steps, washes, images and any steps that you may have changes while troubleshooting.
These details will not only help me to better understand this issue and be able to offer suggestions but will also be used for internal testing of the products if deemed necessary. If we are unable to solve the problems you are experiencing we will replace or refund the antibody according to our Abpromise guarantee.
Answered on Jan 27 2012