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Thank you for your mail.
Please see the below reply against each query in Blue Color on this complaint as desired by you.
The pictures of the controls in which the antibodies have not workedwill be sent to you soon upon its receipt from this customer.
Looking forward to your valued response/ comments on it.
With Best Regards,
Asked on Sep 28 2012
Thank you for your email.
We will look forward to receive the images. On the optimization note we strongly recommend to include the blocking step in protocol; please try 5%BSA or 5-10% normal serum for 1-2 hours at room temperature with species same as secondary antibody. I am sure this step would improve the staining with ab103674 and ab39256.
I may be that the tissue sections you are using does not express the targets GCET1, ERG, CD14 or express at very low level so could you provide the details tissue type and species of tissue section tested. We are interested in knowing e.g. human brain tissue sections or house heart tissue sections etc.
I would suggest trying range of incubation with microwave e.g. for 5, 10, 15 and 20 minutes. This range would help to get the optimized incubation at which the antigen is maximum exposed.
I hope these suggestions would be helpful. Should you have any further question please do not hesitate to contact us.
Answered on Sep 28 2012