Anti-CD14 antibody [5A3B11B5] (ab36595)

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Flow Cytometry - Anti-CD14 antibody [5A3B11B5] (ab36595)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [5A3B11B5] (ab36595)

Key features and details

  • Mouse monoclonal [5A3B11B5] to CD14
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD14 antibody [5A3B11B5]
    See all CD14 primary antibodies

  • Description

    Mouse monoclonal [5A3B11B5] to CD14

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3).

  • Positive control

    • Human lymph node normal tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab36595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 0.01µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 0.01µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

  • Tissue specificity

    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

  • Sequence similarities

    Contains 11 LRR (leucine-rich) repeats.

  • Post-translational
    modifications

    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    Images

    • Flow Cytometry - Anti-CD14 antibody [5A3B11B5] (ab36595)
      Flow Cytometry - Anti-CD14 antibody [5A3B11B5] (ab36595)

      Human peripheral blood cells stained with ab36595 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab36595, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [5A3B11B5] (ab36595)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [5A3B11B5] (ab36595)
      Ab36595 at a 1/1300 dilution (Figure 1), and normal mouse serum at a 1/20 dilution (Figure 2), staining paraffin embedded human normal lymph node by immunohistochemistry. HRP-anti-mouse was used as the secondary antibody before colour development with DAB.

    References (0)

    Publishing research using ab36595? Please let us know so that we can cite the reference in this datasheet.

    ab36595 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Sorry, I forgot to ask prices also for:

    ab36595 and ab49755

    Which can be the best for paraffin embedded eye sections in your experience?

    Thanks again for your help

    Regards

    Read More
    Answer

    Thank you for your further questions.

    I can confirm the following prices:
    CD14 antibody [5A3B11B5] (ab36595) 100 ul £227.00
    CD14 antibody [7] (ab49755) 200 ug £227.00
    Delivery: £16.00 to United Kingdom

    This information can be found on the individual online datasheets. Please ensure toselect the correct countryfrom thedropdown menu at the top right corner of the webpage. The correct price for the product and delivery charge in the UK will then show at the top of the page.

    Regarding which of these antibodies is best for parafin embedded eye sections, I am sorry that neither of these CD14 antibodies have specifically been tested on eye sections as far as we are aware. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our 6 month guarantee in the applications and specieslisted on the datasheets. Therefore, any CD14 antibody tested in thespecies and application you are using should be suitable for your applications. Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

    I hope this will be helpful. Should you have any further questions please do not hesitate to ask.

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