Anti-CD14 antibody [61D3] - Low endotoxin, Azide free (ab171201)

Product name

Anti-CD14 antibody [61D3] - Low endotoxin, Azide free
See all CD14 primary antibodies
Description

Mouse monoclonal [61D3] to CD14 - Low endotoxin, Azide free
Host species

Mouse
Tested applications

Suitable for: Functional Studies, Flow Cytmore details
Species reactivity

Reacts with: Human
Immunogen

The details of the immunogen for this antibody are not available.
Positive control
- Human peripheral blood leukocytes.
General notes

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Constituent: PBS

Aqueous buffer, no sodium azide.
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

61D3
Isotype

IgG1
Light chain type

kappa
Research areas

Our Abpromise guarantee covers the use of ab171201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Functional Studies		Use at an assay dependent concentration.
Flow Cyt		Use 1µg for 10^5-8 cells. (in a final volume of 100 μL). ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Application

Abreviews

Notes

Functional Studies

Use at an assay dependent concentration.

Flow Cyt

Use 1µg for 10^5-8 cells.

(in a final volume of 100 μL).

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Function

Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Tissue specificity

Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Sequence similarities

Contains 11 LRR (leucine-rich) repeats.
Post-translational
modifications

N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P08571 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
- CD14 antigen antibody
- CD14 molecule antibody
- CD14_HUMAN antibody
- LPS-R antibody
- Mo2 antibody
- Monocyte differentiation antigen CD14 antibody
- Monocyte differentiation antigen CD14 urinary form antibody
- Monocyte differentiation antigen CD14, membrane-bound form antibody
- Myeloid cell specific leucine rich glycoprotein antibody
- Myeloid cell-specific leucine-rich glycoprotein antibody
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ab171201 has not yet been referenced specifically in any publications.

Publishing research using ab171201? Please let us know so that we can cite the reference in this datasheet.

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