Anti-CD14 antibody [61D3] (PE/Cy5.5 ®) (ab25390)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-CD14 antibody [61D3] (PE/Cy5.5 ®)
See all CD14 primary antibodies -
Description
Mouse monoclonal [61D3] to CD14 (PE/Cy5.5 ®) -
Host species
Mouse -
Conjugation
PE/Cy5.5 ®. Ex: 496nm, Em: 690nm -
Specificity
Human CD14 -
Tested applications
Suitable for: Flow Cyt, IHC-Fr, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Stabilising agent. -
Concentration information loading... -
Purity
Ascites -
Purification notes
Purified from ascites. -
Clonality
Monoclonal -
Clone number
61D3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab25390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. ab154453 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
Tissue specificity
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
Sequence similarities
Contains 11 LRR (leucine-rich) repeats. -
Post-translational
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
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Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
Datasheets and documents
References
This product has been referenced in:
- Buggert M et al. Limited immune surveillance in lymphoid tissue by cytolytic CD4+ T cells during health and HIV disease. PLoS Pathog 14:e1006973 (2018). Flow Cyt ; Human . Read more (PubMed: 29652923) »
- Mund JA et al. Human proangiogenic circulating hematopoietic stem and progenitor cells promote tumor growth in an orthotopic melanoma xenograft model. Angiogenesis 16:953-62 (2013). Read more (PubMed: 23877751) »
