redFluor™ 710 Anti-CD14 antibody [61D3] (ab253087)
Key features and details
- redFluor™ 710 Mouse monoclonal [61D3] to CD14
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: redFluor™ 710
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
redFluor™ 710 Anti-CD14 antibody [61D3]
See all CD14 primary antibodies -
Description
redFluor™ 710 Mouse monoclonal [61D3] to CD14 -
Host species
Mouse -
Conjugation
redFluor™ 710 -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
-
Positive control
- Flow Cyt: Human peripheral blood monocytes.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
61D3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
Use at 5 µL (0.5 µg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µL. The number of cells within a samaple should be determined empirically, but typically ranges between 1x105 to 1x108 cells. |
Notes |
---|
Flow Cyt
Use at an assay dependent concentration. Use at 5 µL (0.5 µg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µL. The number of cells within a samaple should be determined empirically, but typically ranges between 1x105 to 1x108 cells. |
Target
-
Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
Tissue specificity
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
Sequence similarities
Contains 11 LRR (leucine-rich) repeats. -
Post-translational
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
-
Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab253087 has not yet been referenced specifically in any publications.