Overview

  • Product name

  • Description

    Rabbit polyclonal to CD14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 25-285 of Human CD14 (BC010507).

  • Positive control

    • Human fetal thymus and Hep2 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab106285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 40 kDa.

Target

  • Function

    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificity

    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similarities

    Contains 11 LRR (leucine-rich) repeats.
  • Post-translational
    modifications

    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Images

  • All lanes : Anti-CD14 antibody (ab106285) at 1/1000 dilution

    Lane 1 : Human fetal thymus lysate
    Lane 2 : Hep2 cell lysate

    Predicted band size: 40 kDa

References

This product has been referenced in:

  • Lin Z  et al. Epistatic Association of CD14 and NOTCH2 Genetic Polymorphisms with Biliary Atresia in a Southern Chinese Population. Mol Ther Nucleic Acids 13:590-595 (2018). Read more (PubMed: 30439647) »
  • Sambamurthy N  et al. Deficiency of the pattern-recognition receptor CD14 protects against joint pathology and functional decline in a murine model of osteoarthritis. PLoS One 13:e0206217 (2018). Read more (PubMed: 30485272) »
See all 4 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Question
Answer

Thank you for your telephone enquiry earlier this afternoon.

I can confirm that both these antibodies can be kept in the fridge at 4oC overnight until your colleagues return tomorrow.They will then need to store as directed:

For ab34837 Anti-IL1 beta antibody, as stated on the datasheet, this should be aliquotted and stored as soon as possible.

For ab106285 Anti-CD14 antibody, there is some confusing information on the datasheet which I have asked to be updated. I apologize for any confusion. I can confirm this antibody is sold as a liquid, so does NOT require reconstituting. I can suggest for your colleagues to directly go ahead and aliquot and store this when they return tomorrow.

I hope this will be helpful. Please let me know if you have any further questions.

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Answer

DISCOUNT CODE: ****** (no need to mention this code on the original order for ab106285)
Expiration date: DD MM YYYY

I am very pleased to hear you would like to accept our offer and test ab106285 in Dog. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for Dog and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab106285.

To our knowledge,ab106285 has not been tested in Dog. The homology between the immunogen and the Dog CD14 is 70%, see attachment.

Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. However, I can offer a discount off a future purchase if you buy ab106285 now, test it in Dog and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 freeprimary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab106285 in Dog. I will then send a discount code. This code must be issued before purchasing ab106285 so please wait for my reply before ordering.

2. Purchase ab106285 either by phone, fax, or online (https://www.abcam.com).

3. Test it in Dog.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab106285 turns out to be unsuitable for Dog, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

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Answer

DISCOUNT CODE: 0000
Expiration date: 18. September 2012

I am very pleased to hear you would like to accept our offer and test ab106285 in mouse. This code will give you: the value of ab106285 off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you very much for your interest in ab106285.
I can confirm that ab106285 is tested and guaranteed for IHC-P.
To our knowledge, ab106285 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab106285 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody or value off the next order.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab106285 in mouse. I will then send a discount code. This code must be issued before purchasing ab106285 so please wait for my reply before ordering.
2. Purchase ab106285 either by phone, fax, or online (www.abcam.com).
3. Test it in mouse.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab106285 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

DISCOUNT CODE: xxxxxxxx Expiration date: xxxxxxx I am very pleased to hear you would like to accept our offer and test ab106285 in Rat samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for Rat sampes with IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Specification
Mammary gland
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Irina Teplova

Verified customer

Submitted Jul 19 2011

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