Anti-CD14 antibody (ab203294)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-CD14 antibody
See all CD14 primary antibodies -
Description
Rabbit polyclonal to CD14 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Mouse CD14 aa 240-270 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Sequence:L AAARVQLQGL DLSHNSLRDA AGAPSCDWPS
Database link: P10810 -
Positive control
- Human lung carcinoma tissue; Mouse lung cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab203294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/1000. Predicted molecular weight: 40 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use at 1/50 - 1/200 with fluorescent detection methods. |
Target
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Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
Tissue specificity
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
Sequence similarities
Contains 11 LRR (leucine-rich) repeats. -
Post-translational
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 929 Human
- Entrez Gene: 12475 Mouse
- Entrez Gene: 60350 Rat
- Omim: 158120 Human
- SwissProt: P08571 Human
- SwissProt: P10810 Mouse
- SwissProt: Q63691 Rat
- Unigene: 163867 Human
see all -
Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
Images
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Western blot - Anti-CD14 antibody (ab203294)Anti-CD14 antibody (ab203294) at 1/300 dilution + Mouse lung cell lysate
Predicted band size: 40 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody (ab203294)Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling CD14 with ab203294 at 1/200 dilution, followed by secondary detection and DAB staining.
Datasheets and documents
References
This product has been referenced in:
- Wang YX et al. Porphyromonas gingivalis induces depression via downregulating p75NTR-mediated BDNF maturation in astrocytes. Brain Behav Immun 81:523-534 (2019). Read more (PubMed: 31299366) »
- Zhang J et al. Porphyromonas gingivalis lipopolysaccharide induces cognitive dysfunction, mediated by neuronal inflammation via activation of the TLR4 signaling pathway in C57BL/6 mice. J Neuroinflammation 15:37 (2018). WB . Read more (PubMed: 29426327) »
