Key features and details
- Goat polyclonal to CD14
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD14 antibody
See all CD14 primary antibodies
DescriptionGoat polyclonal to CD14
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human Lymph Node lysate and tonsil lysates. Human spleen tissue sections.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab45870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).
A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042).
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Sequence similaritiesContains 11 LRR (leucine-rich) repeats.
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localizationCell membrane.
- Information by UniProt
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
Anti-CD14 antibody (ab45870) at 1 µg/ml + Human Lymph Node lysate (35µg protein in RIPA buffer).
Predicted band size: 40 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab45870 at 4µg/ml staining CD14 in human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Image shows strong clustered staining in some splenocytes.
ab45870 has been referenced in 8 publications.
- Han Q et al. Application of a TLR overexpression cell model in pyrogen detection. Biotechnol Bioeng 116:1269-1279 (2019). PubMed: 30684361
- Herter S et al. A novel three-dimensional heterotypic spheroid model for the assessment of the activity of cancer immunotherapy agents. Cancer Immunol Immunother 66:129-140 (2017). IHC-P ; Human . PubMed: 27858101
- Satoh-Nakamura T et al. CD14+ follicular dendritic cells in lymphoid follicles may play a role in the pathogenesis of IgG4-related disease. Biomed Res 36:143-53 (2015). PubMed: 25876665
- Li Y & Ding J Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer. Mol Med Rep 12:2169-74 (2015). PubMed: 25845433
- Azakir BA et al. Proteasome inhibitors increase missense mutated dysferlin in patients with muscular dystrophy. Sci Transl Med 6:250ra112 (2014). Human . PubMed: 25143362
- Ciechomska M et al. Toll-like receptor-mediated, enhanced production of profibrotic TIMP-1 in monocytes from patients with systemic sclerosis: role of serum factors. Ann Rheum Dis : (2012). IHC-P ; Human . PubMed: 23223421
- Okuzaki D et al. LeukoCatch, a quick and efficient tool for the preparation of leukocyte extracts from blood. BMC Clin Pathol 11:9 (2011). WB ; Human . PubMed: 21849019
- Petheo GL et al. Molecular and functional characterization of Hv1 proton channel in human granulocytes. PLoS One 5:e14081 (2010). WB ; Human . PubMed: 21124855