Anti-CD14 antibody (ab45870)

Submit a review Q&A (1)References (8)

Key features and details

  • Goat polyclonal to CD14
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CD14 antibody
    See all CD14 primary antibodies

  • Description

    Goat polyclonal to CD14

  • Host species

    Goat

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide:

    C-KRVDADADPRQYAD

    , corresponding to Internal sequence amino acids 71-84 of Human CD14

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human Lymph Node lysate and tonsil lysates. Human spleen tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab45870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).

A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042).
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

  • Function

    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

  • Tissue specificity

    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

  • Sequence similarities

    Contains 11 LRR (leucine-rich) repeats.

  • Post-translational
    modifications

    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    Images

    • Western blot - Anti-CD14 antibody (ab45870)
      Western blot - Anti-CD14 antibody (ab45870)
      Anti-CD14 antibody (ab45870) at 1 µg/ml + Human Lymph Node lysate (35µg protein in RIPA buffer).

      Predicted band size: 40 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?



      Primary incubation was 1 hour. Detected by chemiluminescence.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody (ab45870)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody (ab45870)
      ab45870 at 4µg/ml staining CD14 in human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
      Image shows strong clustered staining in some splenocytes.

    References (8)

    Publishing research using ab45870? Please let us know so that we can cite the reference in this datasheet.

    ab45870 has been referenced in 8 publications.

    • Han Q  et al. Application of a TLR overexpression cell model in pyrogen detection. Biotechnol Bioeng 116:1269-1279 (2019). PubMed: 30684361
    • Herter S  et al. A novel three-dimensional heterotypic spheroid model for the assessment of the activity of cancer immunotherapy agents. Cancer Immunol Immunother 66:129-140 (2017). IHC-P ; Human . PubMed: 27858101
    • Satoh-Nakamura T  et al. CD14+ follicular dendritic cells in lymphoid follicles may play a role in the pathogenesis of IgG4-related disease. Biomed Res 36:143-53 (2015). PubMed: 25876665
    • Li Y & Ding J Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer. Mol Med Rep 12:2169-74 (2015). PubMed: 25845433
    • Azakir BA  et al. Proteasome inhibitors increase missense mutated dysferlin in patients with muscular dystrophy. Sci Transl Med 6:250ra112 (2014). Human . PubMed: 25143362
    • Ciechomska M  et al. Toll-like receptor-mediated, enhanced production of profibrotic TIMP-1 in monocytes from patients with systemic sclerosis: role of serum factors. Ann Rheum Dis : (2012). IHC-P ; Human . PubMed: 23223421
    • Okuzaki D  et al. LeukoCatch, a quick and efficient tool for the preparation of leukocyte extracts from blood. BMC Clin Pathol 11:9 (2011). WB ; Human . PubMed: 21849019
    • Petheo GL  et al. Molecular and functional characterization of Hv1 proton channel in human granulocytes. PLoS One 5:e14081 (2010). WB ; Human . PubMed: 21124855

    Customer reviews and Q&As

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    Question

    ab78313 is not working on human skin cells in IHC, acetone fixed and paraffin embedded sections. As this product is no longer available I would like to receive ab45870 as a replacement.

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    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with product ab45870. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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