Anti-CD14 antibody (ab45870)

Submit a review Q&A (1)References (9)
Western blot - Anti-CD14 antibody (ab45870)

    Key features and details

    • Goat polyclonal to CD14
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-CD14 antibody [EPR3653] (ab133335)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-CD14 antibody
      See all CD14 primary antibodies

    • Description

      Goat polyclonal to CD14

    • Host species

      Goat

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Synthetic peptide within Human CD14 aa 50-150 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • WB: Human blood mononucleocyte lysate.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab45870 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).

    A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042).
    Notes
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).

    A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042).

    Target

    • Function

      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

    • Tissue specificity

      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

    • Sequence similarities

      Contains 11 LRR (leucine-rich) repeats.

    • Post-translational
      modifications

      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 14 antibody
        • CD_antigen=CD14 antibody
        • CD14 antibody
        • CD14 antigen antibody
        • CD14 molecule antibody
        • CD14_HUMAN antibody
        • LPS-R antibody
        • Mo2 antibody
        • Monocyte differentiation antigen CD14 antibody
        • Monocyte differentiation antigen CD14 urinary form antibody
        • Monocyte differentiation antigen CD14, membrane-bound form antibody
        • Myeloid cell specific leucine rich glycoprotein antibody
        • Myeloid cell-specific leucine-rich glycoprotein antibody
        see all

      Images

      • Western blot - Anti-CD14 antibody (ab45870)
        Western blot - Anti-CD14 antibody (ab45870)
        All lanes : Anti-CD14 antibody (ab45870) at 1 µg/ml

        Lane 1 : Human Peripheral Blood Mononucleocyte lysate
        Lane 2 : Human Peripheral Blood Mononucleocyte lysate with Immunizing peptide

        Lysates/proteins at 35 µg per lane.

        Predicted band size: 40 kDa
        Observed band size: 55 kDa why is the actual band size different from the predicted?
        Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands.



        The observed molecular weight corresponds to the glycosylated form.

        Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

      References (9)

      Publishing research using ab45870? Please let us know so that we can cite the reference in this datasheet.

      ab45870 has been referenced in 9 publications.

      • Liu M  et al. Myeloid-derived suppressor cells in gastroenteropancreatic neuroendocrine neoplasms. Endocrine 71:242-252 (2021). PubMed: 33010002
      • Han Q  et al. Application of a TLR overexpression cell model in pyrogen detection. Biotechnol Bioeng 116:1269-1279 (2019). PubMed: 30684361
      • Herter S  et al. A novel three-dimensional heterotypic spheroid model for the assessment of the activity of cancer immunotherapy agents. Cancer Immunol Immunother 66:129-140 (2017). IHC-P ; Human . PubMed: 27858101
      • Satoh-Nakamura T  et al. CD14+ follicular dendritic cells in lymphoid follicles may play a role in the pathogenesis of IgG4-related disease. Biomed Res 36:143-53 (2015). PubMed: 25876665
      • Li Y & Ding J Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer. Mol Med Rep 12:2169-74 (2015). PubMed: 25845433
      • Azakir BA  et al. Proteasome inhibitors increase missense mutated dysferlin in patients with muscular dystrophy. Sci Transl Med 6:250ra112 (2014). Human . PubMed: 25143362
      • Ciechomska M  et al. Toll-like receptor-mediated, enhanced production of profibrotic TIMP-1 in monocytes from patients with systemic sclerosis: role of serum factors. Ann Rheum Dis : (2012). IHC-P ; Human . PubMed: 23223421
      • Okuzaki D  et al. LeukoCatch, a quick and efficient tool for the preparation of leukocyte extracts from blood. BMC Clin Pathol 11:9 (2011). WB ; Human . PubMed: 21849019
      • Petheo GL  et al. Molecular and functional characterization of Hv1 proton channel in human granulocytes. PLoS One 5:e14081 (2010). WB ; Human . PubMed: 21124855

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      ab78313 is not working on human skin cells in IHC, acetone fixed and paraffin embedded sections. As this product is no longer available I would like to receive ab45870 as a replacement.

      Read More
      Answer

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with product ab45870. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com