Name: Anti-CD14 antibody [EPR3653]
Brand: RabMAb
SKU: ab133335
Rating: 4 (1 reviews)

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This product Rabbit Anti-CD14 antibody [EPR3653] (ab133335)
WB, IHC-P

Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

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Product name

Anti-CD14 antibody [EPR3653]
See all CD14 primary antibodies
Description

Rabbit monoclonal [EPR3653] to CD14
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human CD14 aa 300-400. The exact sequence is proprietary.
Positive control
- IHC-P: human colon, placenta and tonsil tissue; WB: Human tonsi, SW480 and PBMCl lysate.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (K_D)

K_D = 4.70 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3653
Isotype

IgG
Research areas

Alternative Versions
- Anti-CD14 antibody [EPR3653] (HRP) (ab195525)
- Anti-CD14 antibody [EPR3653] - Low endotoxin, Azide free (ab214438)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- SW480 whole cell lysate (ab3957)
Recombinant Protein
- Recombinant Mouse CD14 protein (ab207103)

Our Abpromise guarantee covers the use of ab133335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 40 kDa).

IHC-P

1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/500 - 1/1000.

Function

Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Tissue specificity

Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Sequence similarities

Contains 11 LRR (leucine-rich) repeats.
Post-translational
modifications

N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P08571 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
- CD14 antigen antibody
- CD14 molecule antibody
- CD14_HUMAN antibody
- LPS-R antibody
- Mo2 antibody
- Monocyte differentiation antigen CD14 antibody
- Monocyte differentiation antigen CD14 urinary form antibody
- Monocyte differentiation antigen CD14, membrane-bound form antibody
- Myeloid cell specific leucine rich glycoprotein antibody
- Myeloid cell-specific leucine-rich glycoprotein antibody
see all

Western blot - Anti-CD14 antibody [EPR3653] (ab133335)

Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (purified) + His-Tagged Human CD14 (aa20 to 345) recombinant protein at 0.015 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 40 kDa
Observed band size: 43 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking and diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CD14 with purified ab133335 at 1/2000 dilution (0.04 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-CD14 antibody [EPR3653] (ab133335)

All lanes : Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (purified)

Lane 1 : Human tonsil tissue lysate
Lane 2 : HeLa (Human cervix adenocarcinoma) whole cell lysate
Lane 3 : U-937 (Human histiocytic lymphoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 40 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and diluting buffer: 5% NFDM/TBST

The expression level in HeLa and U-937 are low (PMID: 9886426 and 15730927)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

Immunohistochemical analysis of Formalin-fixed, paraffin- embedded human tonsil tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution. No blocking step performed. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human tonsil tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human placenta tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution.
Western blot - Anti-CD14 antibody [EPR3653] (ab133335)

All lanes : Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (unpurified)

Lane 1 : PBMC cell lysate
Lane 2 : Human tonsil cell lysate
Lane 3 : SW480 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

Predicted band size: 40 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Other - Anti-CD14 antibody [EPR3653] (ab133335)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Datasheet

This product has been referenced in:

Jamaly S et al. Elevated plasma levels of P-selectin glycoprotein ligand-1-positive microvesicles in patients with unprovoked venous thromboembolism. J Thromb Haemost N/A:N/A (2018). Read more (PubMed: 29851269) »
Chakraborty B et al. Deciphering genetic regulation of CD14 by SP1 through characterization of peripheral blood mononuclear transcriptome of P. faiciparum and P. vivax infected malaria patients. EBioMedicine 37:442-452 (2018). Read more (PubMed: 30337251) »

See all 7 Publications for this product

Publishing research using ab133335? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer ph 8,5

Sample

Human Tissue sections (spleen)

Specification

spleen

Fixative

Paraformaldehyde

Mr. Rudolf Jung

Verified customer

Submitted Oct 22 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Recombinant Anti-CD14 antibody [EPR3653] (ab133335)

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