For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    cd14-antibody-epr3653-ab133335.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Cell Type Markers CD Myeloid Cells
Share by email
RecombinantRabMAb

Recombinant Anti-CD14 antibody [EPR3653] (ab133335)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (12)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
  • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
  • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
  • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
  • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
  • OI-RD Scanning - Anti-CD14 antibody [EPR3653] (ab133335)
  • Anti-CD14 antibody [EPR3653] (ab133335)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3653] to CD14
  • Suitable for: WB, IHC-P
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Mouse CD14 protein (ab207103)

View more associated products

Overview

  • Product name

    Anti-CD14 antibody [EPR3653]
    See all CD14 primary antibodies
  • Description

    Rabbit monoclonal [EPR3653] to CD14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: human colon, placenta and tonsil tissue; WB: Human tonsil tissue lysate, SW480 and PBMCl lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 4.70 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3653
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • CD markers ELISA kits

Associated products

  • Alternative Versions

    • HRP Anti-CD14 antibody [EPR3653] (ab195525)
    • Anti-CD14 antibody [EPR3653] - Low endotoxin, Azide free (ab214438)
    • Anti-CD14 antibody [EPR3653] - BSA and Azide free (ab226121)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • SW480 whole cell lysate (ab3957)
  • Recombinant Protein

    • Recombinant Mouse CD14 protein (ab207103)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 40 kDa).
IHC-P (1)
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/500 - 1/1000.

Notes
WB
1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 40 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/500 - 1/1000.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
    • Tissue specificity

      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
    • Sequence similarities

      Contains 11 LRR (leucine-rich) repeats.
    • Post-translational
      modifications

      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P08571 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 929 Human
      • Omim: 158120 Human
      • SwissProt: P08571 Human
      • Unigene: 163867 Human
      • Alternative names

        • CD 14 antibody
        • CD_antigen=CD14 antibody
        • CD14 antibody
        • CD14 antigen antibody
        • CD14 molecule antibody
        • CD14_HUMAN antibody
        • LPS-R antibody
        • Mo2 antibody
        • Monocyte differentiation antigen CD14 antibody
        • Monocyte differentiation antigen CD14 urinary form antibody
        • Monocyte differentiation antigen CD14, membrane-bound form antibody
        • Myeloid cell specific leucine rich glycoprotein antibody
        • Myeloid cell-specific leucine-rich glycoprotein antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CD14 with purified ab133335 at 1/2000 dilution (0.04 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
        Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
        Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (purified) + His-Tagged Human CD14 (aa20 to 345) recombinant protein at 0.015 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 40 kDa
        Observed band size: 43 kDa why is the actual band size different from the predicted?


        Exposure time: 1 second


        Blocking and diluting buffer: 5% NFDM/TBST

      • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
        Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
        All lanes : Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution

        Lane 1 : Human tonsil lysates prepared in RIPA lysis method
        Lane 2 : Human tonsil lysates prepared in 1%SDS Hot lysis method

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 40 kDa


        Exposure time: 45 seconds


        Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
        Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
        All lanes : Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (purified)

        Lane 1 : Human tonsil tissue lysate prepared in 1% SDS Hot lysis method
        Lane 2 : HeLa (Human cervix adenocarcinoma) whole cell lysate
        Lane 3 : U-937 (Human histiocytic lymphoma) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        Lane 1 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
        Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 40 kDa
        Observed band size: 53 kDa why is the actual band size different from the predicted?


        Exposure time: 3 minutes


        Blocking and diluting buffer: 5% NFDM/TBST

        The expression level in HeLa and U-937 are low (PMID: 9886426 and 15730927)

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

        Immunohistochemical analysis of Formalin-fixed, paraffin- embedded human tonsil tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution. No blocking step performed. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

        Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human tonsil tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653] (ab133335)

        Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human placenta tissue labelling CD14 with ab133335 (unpurified) at 1/500 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

      • Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
        Western blot - Anti-CD14 antibody [EPR3653] (ab133335)
        All lanes : Anti-CD14 antibody [EPR3653] (ab133335) at 1/1000 dilution (unpurified)

        Lane 1 : PBMC cell lysate prepared in 1% SDS Hot lysis method
        Lane 2 : Human tonsil tissue lysate prepared in 1%SDS Hot lysis method
        Lane 3 : SW480 cell lysate prepared in 1%SDS Hot lysis method

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 40 kDa
        Observed band size: 53 kDa why is the actual band size different from the predicted?

      • OI-RD Scanning - Anti-CD14 antibody [EPR3653] (ab133335)
        OI-RD Scanning - Anti-CD14 antibody [EPR3653] (ab133335)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-CD14 antibody [EPR3653] (ab133335)
        Anti-CD14 antibody [EPR3653] (ab133335)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (12)

      Publishing research using ab133335? Please let us know so that we can cite the reference in this datasheet.

      ab133335 has been referenced in 12 publications.

      • Feng Y  et al. LncRNA NR-104098 Inhibits AML Proliferation and Induces Differentiation Through Repressing EZH2 Transcription by Interacting With E2F1. Front Cell Dev Biol 8:142 (2020). PubMed: 32296698
      • Chang HF  et al. Investigation of Cytotoxic T Lymphocyte Function during Allorejection in the Anterior Chamber of the Eye. Int J Mol Sci 21:N/A (2020). PubMed: 32629968
      • Hasegawa T  et al. Reduction in Human Epidermal Langerhans Cells with Age Is Associated with Decline in CXCL14-Mediated Recruitment of CD14+ Monocytes. J Invest Dermatol 140:1327-1334 (2020). PubMed: 31881212
      • Wheeler MA  et al. Environmental Control of Astrocyte Pathogenic Activities in CNS Inflammation. Cell 176:581-596.e18 (2019). PubMed: 30661753
      • Jamaly S  et al. Elevated plasma levels of P-selectin glycoprotein ligand-1-positive microvesicles in patients with unprovoked venous thromboembolism. J Thromb Haemost N/A:N/A (2018). PubMed: 29851269
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review

      Filter by Application

      Filter by Species

      Filter by Ratings

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD14 antibody [EPR3653]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer ph 8,5
      Sample
      Human Tissue sections (spleen)
      Specification
      spleen
      Fixative
      Paraformaldehyde
      Read More

      Mr. Rudolf Jung

      Verified customer

      Submitted Oct 22 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.