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    cd14-antibody-lpsr2408-ab238091.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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Anti-CD14 antibody [LPSR/2408] (ab238091)

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Protein Array - Anti-CD14 antibody [LPSR/2408] (ab238091)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [LPSR/2408] (ab238091)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [LPSR/2408] (ab238091)

Key features and details

  • Mouse monoclonal [LPSR/2408] to CD14
  • Suitable for: Protein Array, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD14 antibody [LPSR/2408]
    See all CD14 primary antibodies
  • Description

    Mouse monoclonal [LPSR/2408] to CD14
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD14 aa 25-148. The exact sequence is proprietary.
    Database link: P08571

  • Positive control

    • IHC-P: Human lymph node and tonsil tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    LPSR/2408
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • CD markers ELISA kits
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP kits

Associated products

  • Alternative Versions

    • Anti-CD14 antibody [LPSR/2408] - BSA and Azide free (ab237945)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Mouse CD14 protein (ab207103)
  • Related Products

    • Recombinant human CD14 protein (ab167706)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab238091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature.

Notes
Protein Array
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature.

Target

  • Function

    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificity

    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similarities

    Contains 11 LRR (leucine-rich) repeats.
  • Post-translational
    modifications

    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P08571 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 929 Human
    • Omim: 158120 Human
    • SwissProt: P08571 Human
    • Unigene: 163867 Human
    • Alternative names

      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    Images

    • Protein Array - Anti-CD14 antibody [LPSR/2408] (ab238091)
      Protein Array - Anti-CD14 antibody [LPSR/2408] (ab238091)
      ab238091 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [LPSR/2408] (ab238091)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [LPSR/2408] (ab238091)

      Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD14 using ab238091 at 2 µg/ml in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [LPSR/2408] (ab238091)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [LPSR/2408] (ab238091)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD14 using ab238091 at 2 µg/ml in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab238091? Please let us know so that we can cite the reference in this datasheet.

    ab238091 has not yet been referenced specifically in any publications.

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