PE/Cy7® Anti-CD14 antibody [MEM-15] (ab234214)
Key features and details
- PE/Cy7® Mouse monoclonal [MEM-15] to CD14
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Isotype: IgG1
Overview
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Product name
PE/Cy7® Anti-CD14 antibody [MEM-15]
See all CD14 primary antibodies -
Description
PE/Cy7® Mouse monoclonal [MEM-15] to CD14 -
Host species
Mouse -
Conjugation
PE/Cy7®. Ex: 496nm, Em: 774nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Full length protein corresponding to Human CD14. A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
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Positive control
- Flow Cytomtery: Human peripheral blood cells.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
ab234214 is conjugated with tandem dye PE-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
Clonality
Monoclonal -
Clone number
MEM-15 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP/Cy5.5® Anti-CD14 antibody [MEM-15], prediluted (ab157312)
- APC/Cy7® Anti-CD14 antibody [MEM-15], prediluted (ab194805)
- PE/Cy5® Anti-CD14 antibody [MEM-15] (ab232910)
- Alexa Fluor® 700 Anti-CD14 antibody [MEM-15] (ab269326)
- PerCP Anti-CD14 antibody [MEM-15] (ab269327)
- FITC Anti-CD14 antibody [MEM-15] (ab28061)
- Biotin Anti-CD14 antibody [MEM-15] (ab28118)
- APC Anti-CD14 antibody [MEM-15] (ab60901)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 4µl for 106 cells.
(or 100 µL whole blood) |
Notes |
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Flow Cyt
Use 4µl for 106 cells. (or 100 µL whole blood) |
Target
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Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
Tissue specificity
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
Sequence similarities
Contains 11 LRR (leucine-rich) repeats. -
Post-translational
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
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Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab234214 has not yet been referenced specifically in any publications.