Key features and details
- Mouse monoclonal [MEM-18] to CD14 (Biotin)
- Suitable for: Flow Cyt, IP, WB, ELISA, Functional Studies
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Product nameAnti-CD14 antibody [MEM-18] (Biotin)
See all CD14 primary antibodies
DescriptionMouse monoclonal [MEM-18] to CD14 (Biotin)
Tested applicationsSuitable for: Flow Cyt, IP, WB, ELISA, Functional Studiesmore details
Species reactivityReacts with: Human
Predicted to work with: Non human primates
A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab21889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/1 - 1/1000.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.|
|ELISA||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Sequence similaritiesContains 11 LRR (leucine-rich) repeats.
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localizationCell membrane.
- Information by UniProt
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
ab21889 has not yet been referenced specifically in any publications.