Key features and details
- Mouse monoclonal [MEM-18] to CD14 - Low endotoxin, Azide free
- Suitable for: Flow Cyt, IP, WB, ELISA, Functional Studies
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD14 antibody [MEM-18] - Low endotoxin, Azide free
See all CD14 primary antibodies
DescriptionMouse monoclonal [MEM-18] to CD14 - Low endotoxin, Azide free
Tested applicationsSuitable for: Flow Cyt, IP, WB, ELISA, Functional Studiesmore details
Species reactivityReacts with: Human
Predicted to work with: Monkey, Non human primates
A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab6083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.01µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 45 kDa.|
|ELISA||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration. Blocks binding of bacterial LPS to the monocyte surface.|
FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Sequence similaritiesContains 11 LRR (leucine-rich) repeats.
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localizationCell membrane.
- Information by UniProt
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
Human peripheral blood cells stained with ab6083 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab6083, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab6083 has been referenced in 8 publications.
- van Montfoort N et al. Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism. J Virol 90:6187-99 (2016). PubMed: 27099316
- Landa-Solís C et al. Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications. Cell Tissue Bank : (2015). IF . PubMed: 26220398
- Suzuki K et al. Human anti-microbial cathelicidin peptide LL-37 suppresses the LPS-induced apoptosis of endothelial cells. Int Immunol 23:185-93 (2011). PubMed: 21393634
- He J et al. Mycoplasma genitalium-derived lipid-associated membrane proteins activate NF-kappaB through toll-like receptors 1, 2, and 6 and CD14 in a MyD88-dependent pathway. Clin Vaccine Immunol 16:1750-7 (2009). PubMed: 19793902
- Maurice S et al. Isolation of progenitor cells from cord blood using adhesion matrices. Cytotechnology 54:121-33 (2007). PubMed: 19003027
- Sundan A et al. Soluble CD14 from urine copurifies with a potent inducer of cytokines. Eur J Immunol 24:1779-84 (1994). PubMed: 7519994
- Bazil V et al. Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14. Mol Immunol 26:657-62 (1989). PubMed: 2779588
- Bazil V et al. Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen. Eur J Immunol 16:1583-9 (1986). PubMed: 3493149