Recombinant
RabMAb

Recombinant Anti-CD14 antibody [SP192] (ab183322)

Rabbit recombinant monoclonal CD14 antibody [SP192]. Validated in WB, IHC, Flow Cyt and tested in Human. Cited in 2 publication(s). Independently reviewed in 3 review(s).

Overview

  • Product name
    Anti-CD14 antibody [SP192]
    See all CD14 primary antibodies
  • Description
    Rabbit monoclonal [SP192] to CD14
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD14 aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: P08571

  • Positive control
    • IHC-p: Human tonsil lysate; Human tonsil, Human thymus, Human colon, Human colon adenocarcinoma, Human spleen, Human lung, Human liver, Human esophagus, Human endometrium, Human HK lymphoma, Human B cell lymphoma and Human bladder tissues. FC: PBMC
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

WB 1/100. Predicted molecular weight: 40 kDa.

Target

  • Function
    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificity
    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similarities
    Contains 11 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling CD14 with ab183322 at 1/100 dilution (1.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on stromal cells in the human colon, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183322 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD14 with ab183322 at 1/100 dilution (1.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183322 for 10 mins at room temperature.

  • Flow Cytometry analysis of human PBMC (human peripheral blood mononuclear cell) cells labeling CD14 with purified ab183322 at 1:200 dilution (0.805 µg/ml) - Red. A Goat anti rabbit IgG (Dylight® 488, ab98462) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
  • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human bone marrow tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human colon adenocarcinoma tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human spleen tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human lung tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human esophagus tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human endometrium tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human HK lymphoma tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human B cell lymphoma tissue labeling CD14 with ab183322 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling CD14 with ab183322 at 1/100.

  • Anti-CD14 antibody [SP192] (ab183322) at 1/100 dilution + Human tonsil lysate

    Predicted band size: 40 kDa

References

This product has been referenced in:
  • Bajpai G  et al. The human heart contains distinct macrophage subsets with divergent origins and functions. Nat Med 24:1234-1245 (2018). Read more (PubMed: 29892064) »
  • Takahashi Y  et al. Monocyte chemoattractant protein 1 expression and proliferation in primary central nervous system lymphoma. Oncol Lett 14:264-270 (2017). Read more (PubMed: 28693163) »
See all 3 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
colon
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 18 2019

Application
Immunohistochemistry free floating
Sample
Human Tissue sections (Human inflammed lung tissue)
Specification
Human inflammed lung tissue

Jim Hsiao

Verified customer

Submitted Jan 06 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal)
Antigen retrieval step
Heat mediated
Specification
Colorectal
Blocking step
(agent) for 20 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Christopher Bagnall

Verified customer

Submitted Sep 13 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Colon)
Specification
Colon
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 08 2017

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