Recombinant Anti-CD14 antibody [SP192] (ab183322)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP192] to CD14
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD14 antibody [SP192]
See all CD14 primary antibodies -
Description
Rabbit monoclonal [SP192] to CD14 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-p: Human tonsil lysate; Human tonsil, Human thymus, Human colon, Human colon adenocarcinoma, Human spleen, Human lung, Human liver, Human esophagus, Human endometrium, Human HK lymphoma, Human B cell lymphoma and Human bladder tissues. Flow Cyt: PBMC
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP192 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/200.
|
|
IHC-P | (2) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. |
WB |
1/100. Predicted molecular weight: 40 kDa.
|
Notes |
---|
Flow Cyt
1/200. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. |
WB
1/100. Predicted molecular weight: 40 kDa. |
Target
-
Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
Tissue specificity
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
Sequence similarities
Contains 11 LRR (leucine-rich) repeats. -
Post-translational
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
-
Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
Images
-
Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil labelling CD14 with ab230903 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab220903 anti CD14 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation (ab230903).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling CD14 with ab183322 at 1/100 dilution (1.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on stromal cells in the human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183322 for 10 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD14 with ab183322 at 1/100 dilution (1.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183322 for 10 mins at room temperature. -
Flow Cytometry analysis of human PBMC (human peripheral blood mononuclear cell) cells labeling CD14 with purified ab183322 at 1:200 dilution (0.805 µg/ml) - Red. A Goat anti rabbit IgG (Dylight® 488, ab98462) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
-
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human bone marrow tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human colon adenocarcinoma tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human spleen tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human lung tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human esophagus tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human endometrium tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human HK lymphoma tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human B cell lymphoma tissue labeling CD14 with ab183322 at 1/100.
-
Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling CD14 with ab183322 at 1/100.
-
Anti-CD14 antibody [SP192] (ab183322) at 1/100 dilution + Human tonsil lysate
Predicted band size: 40 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (19)
ab183322 has been referenced in 19 publications.
- Li X LncRNA metastasis-associated lung adenocarcinoma transcript-1 promotes osteogenic differentiation of bone marrow stem cells and inhibits osteoclastic differentiation of Mø in osteoporosis via the miR-124-3p/IGF2BP1/Wnt/β-catenin axis. J Tissue Eng Regen Med 16:311-329 (2022). PubMed: 34962086
- Liu Y et al. Immune phenotypic linkage between colorectal cancer and liver metastasis. Cancer Cell 40:424-437.e5 (2022). PubMed: 35303421
- Shen P et al. Mechanical stress protects against chondrocyte pyroptosis through lipoxin A4 via synovial macrophage M2 subtype polarization in an osteoarthritis model. Biomed Pharmacother 153:113361 (2022). PubMed: 35797941
- Huang X et al. The molecular, immune features, and risk score construction of intraductal papillary mucinous neoplasm patients. Front Mol Biosci 9:887887 (2022). PubMed: 36090038
- Liu M et al. Clinical implications of immune checkpoint markers and immune infiltrates in patients with thymic neuroendocrine neoplasms. Front Oncol 12:917743 (2022). PubMed: 36203421