Overview

  • Product name

    Anti-CD14 antibody [Tuk4]
    See all CD14 primary antibodies
  • Description

    Mouse monoclonal [Tuk4] to CD14
  • Host species

    Mouse
  • Specificity

    Ab27545 recognises CD14 surface antigen, expressed strongly by most peripheral blood monocytes and weakly on granulocytes.
  • Tested applications

    Suitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity

    Reacts with: Sheep, Rabbit, Goat, Cow, Cat, Dog, Human, Pig, Mink, Cynomolgus monkey
  • General notes

    Clone Tuk4 has been shown to block SDF induced chemotaxis of U937 cells in a dose dependent manner. This antibody could therefore be recommended for this purpose.

Properties

Applications

Our Abpromise guarantee covers the use of ab27545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.

Use under reducing conditions.

ELISA Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/200.

Use 5µl of the suggested working dilution to label 106 cells or 100µl whole blood.

 

 

 

 

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificity

    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similarities

    Contains 11 LRR (leucine-rich) repeats.
  • Post-translational
    modifications

    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

References

This product has been referenced in:

  • Slobedman B  et al. Latent cytomegalovirus down-regulates major histocompatibility complex class II expression on myeloid progenitors. Blood 100:2867-73 (2002). Read more (PubMed: 12351397) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (normal dog spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid / Tris EDTA
Permeabilization
No
Specification
normal dog spleen
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 18 2015

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