Immunohistochemistry (Frozen sections) abreview for Anti-CD146 antibody [EPR3208]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen)
Specification
Spleen
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/250
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/250

Additional data

Additional Notes
The trabecular vein of speleen was stained. In addition, this antibldy was also specific to some of T cells (not in this picture). Nucleus stained with DAPI

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Submitted Mar 09 2010

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