Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD146 antibody [EPR3208]

Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (tooth)
Specification
tooth
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/400

Secondary antibody

Name
Non-Abcam antibody was used: HRP labeled polymer anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
We don't use the antigen retrieval step because tooth tissue was detached from the slide surface during the antigen retrieval step.

Abcam user community

Verified customer

Submitted Apr 04 2014

Sign up