Western blot abreview for Anti-CD146 antibody [EPR3208]

Average
Abreviews
Application
Western blot
Loading amount
37.5 µg
Gel Running Conditions
Reduced Denaturing (7% tris acetate gel)
Sample
Human Cell lysate - whole cell (Renal Cancer Cell Line 786-0)
Specification
Renal Cancer Cell Line 786-0
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 5% Milk
Dilution
1/10000

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit hrp
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Bands
Specific: 72 kDa Non-specific: 160, 85, 76 kDa
Exposure
20 minute(s) and 0 second(s)

Additional data

Additional Notes
Markers on image are 225, 150, 100 and 76kDa (shown as a cross)

Dr. Victoria Bridgeman

Verified customer

Submitted May 13 2014

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