Immunohistochemistry (Frozen sections) abreview for Anti-CD146 antibody [EPR3208]

Inconclusive
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Rabbit Alexa Fluor 555 conjugated
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
Tested conditions: 1) 1:100 1:250 1:500 without triton 2) 1:100 1:250 1:500 without triton+ 1Ab at 37C for 5h+streptavidin enhancement Dilutions refer to primary antibody
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

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Submitted Sep 28 2016

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