Immunocytochemistry/ Immunofluorescence abreview for Anti-CD146 antibody [EPR3208]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human brain pericytes)
Permeabilization
Yes - Triton X-100
Specification
Human brain pericytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA with 0.1% Triton in PBS
Dilution
1/300

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 594

Additional data

Additional Notes
The primary antibody was used at a dilution of 1 in 300 and the secondary antibody (Alexa Fluor conjugated Donkey anti-rabbit 594) was used at 1 in 500 dilution.

Radhika Menon

Verified customer

Submitted Jul 06 2017

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