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    cd146-antibody-muc181130-ab233923.pdf

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Immunology Cell Type Markers CD Endothelial Cells
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Anti-CD146 antibody [MUC18/1130] (ab233923)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [MUC18/1130] (ab233923)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [MUC18/1130] (ab233923)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [MUC18/1130] (ab233923)

Key features and details

  • Mouse monoclonal [MUC18/1130] to CD146
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Protein
Product image
Recombinant Human CD146 protein (ab114180)
Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-CD146 antibody [MUC18/1130]
    See all CD146 primary antibodies
  • Description

    Mouse monoclonal [MUC18/1130] to CD146
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human CD146.
    Database link: P43121

  • Positive control

    • IHC-p; Human tonsil and melanoma tissues.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    MUC18/1130
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Endothelial Cells
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human CD146 protein (ab114180)
  • Related Products

    • Recombinant Human CD146 protein (ab114180)

Applications

Our Abpromise guarantee covers the use of ab233923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 min at RT).

Target

  • Function

    Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
  • Tissue specificity

    Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P43121 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4162 Human
    • Omim: 155735 Human
    • SwissProt: P43121 Human
    • Unigene: 599039 Human
    • Alternative names

      • A32 antigen antibody
      • CD 146 antibody
      • CD146 antibody
      • CD146 antigen antibody
      • Cell surface glycoprotein MUC18 antibody
      • Cell surface glycoprotein P1H12 antibody
      • Gicerin antibody
      • Mcam antibody
      • Melanoma adhesion molecule antibody
      • Melanoma associated antigen A32 antibody
      • Melanoma associated antigen MUC18 antibody
      • Melanoma associated glycoprotein MUC18 antibody
      • Melanoma cell adhesion molecule antibody
      • Melanoma-associated antigen A32 antibody
      • Melanoma-associated antigen MUC18 antibody
      • MelCAM antibody
      • MUC 18 antibody
      • MUC18 antibody
      • MUC18_HUMAN antibody
      • S endo 1 antibody
      • S endo 1 endothelial associated antigen antibody
      • S-endo 1 endothelial-associated antigen antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [MUC18/1130] (ab233923)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [MUC18/1130] (ab233923)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD146 using ab233923 at 1 µg/ml in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [MUC18/1130] (ab233923)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [MUC18/1130] (ab233923)

      Formalin-fixed, paraffin-embedded human Melanoma tissue stained for CD146 using ab233923 at 1 µg/ml in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [MUC18/1130] (ab233923)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody [MUC18/1130] (ab233923)

      Formalin-fixed, paraffin-embedded human melanoma tissue stained for CD146 using ab233923 at 1 µg/ml in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab233923? Please let us know so that we can cite the reference in this datasheet.

    ab233923 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab233923.
    Please use the links above to contact us or submit feedback about this product.

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