Anti-CD147 antibody [10E10] (ab230921)

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Overview

  • Product name

    Anti-CD147 antibody [10E10]
    See all CD147 primary antibodies

  • Description

    Mouse monoclonal [10E10] to CD147

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Dog, Monkey

  • Immunogen

    Recombinant full length protein corresponding to Human CD147. (NP_001719). Produced in HEK293T cell.
    Database link: P35613

  • Positive control

    • WB: HEK-293T, HepG2, HeLa, HT-29, A549, COS-7, Jurkat, MDCK, PC-12 and MCF7 whole cell lysate. IHC-P: Human brain and placenta tissue. Flow: HeLa and Jurkat cells. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000.
Flow Cyt 1/100.
ICC/IF 1/100.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function

    Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.

  • Tissue specificity

    Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    N-glycosylated.

  • Cellular localization

    Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • 5A11 antigen antibody
      • 5F7 antibody
      • BASI_HUMAN antibody
      • Basigin (Ok blood group) antibody
      • Basigin antibody
      • Blood brain barrier HT7 antigen antibody
      • Bsg antibody
      • CD 147 antibody
      • CD147 antibody
      • CD147 antigen antibody
      • Collagenase stimulatory factor antibody
      • EMMPRIN antibody
      • Extracellular matrix metalloproteinase inducer antibody
      • Leukocyte activation antigen M6 antibody
      • M 6 antibody
      • M6 antibody
      • M6 leukocyte activation antigen antibody
      • Neurothelin antibody
      • OK antibody
      • OK blood group antibody
      • OK blood group antigen antibody
      • TCSF antibody
      • Tumor cell derived collagenase stimulatory factor antibody
      • Tumor cell-derived collagenase stimulatory factor antibody
      see all

    Images

    • Western blot - Anti-CD147 antibody [10E10] (ab230921)
      Western blot - Anti-CD147 antibody [10E10] (ab230921)
      All lanes : Anti-CD147 antibody [10E10] (ab230921) at 1/500 dilution

      Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : pCMV6-ENTRY CD147-transfected HEK-293T whole cell lysate

      Lysates/proteins at 5 µg per lane.

      Developed using the ECL technique.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (ab230921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (ab230921)

      Formalin-fixed, paraffin-embedded human brain tissue stained for CD147 using ab230921 at 10 μg/ml in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (ab230921)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [10E10] (ab230921)

      Formalin-fixed, paraffin-embedded human placenta tissue stained for CD147 using ab230921 at 10 μg/ml in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [10E10] (ab230921)
      Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [10E10] (ab230921)

      HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD147 (green) using ab230921 at 1/100 dilution in ICC/IF.

    • Western blot - Anti-CD147 antibody [10E10] (ab230921)
      Western blot - Anti-CD147 antibody [10E10] (ab230921)
      All lanes : Anti-CD147 antibody [10E10] (ab230921) at 1/500 dilution

      Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
      Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
      Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
      Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 7 : MDCK (canine kidney cell line) whole cell lysate
      Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
      Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 35 µg per lane.

      Developed using the ECL technique.
    • Flow Cytometry - Anti-CD147 antibody [10E10] (ab230921)
      Flow Cytometry - Anti-CD147 antibody [10E10] (ab230921)

      Flow cytometric analysis of HeLa (human T cell leukemia cell line from peripheral blood) cell line labeling CD147 with ab230921 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

    • Flow Cytometry - Anti-CD147 antibody [10E10] (ab230921)
      Flow Cytometry - Anti-CD147 antibody [10E10] (ab230921)

      Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD147 with ab230921 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

    References

    ab230921 has not yet been referenced specifically in any publications.

    Publishing research using ab230921? Please let us know so that we can cite the reference in this datasheet.

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