Key features and details
- Mouse monoclonal [8D6] to CD147
- Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD147 antibody [8D6]
See all CD147 primary antibodies
DescriptionMouse monoclonal [8D6] to CD147
Specificityab194401 recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147.
Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, ICC/IF, IP, Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human CD147. Human T cell line Molt 13
Database link: P35613
- IHC-P: human renal cell carcinoma tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from bioreactor concentrate.
Our Abpromise guarantee covers the use of ab194401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 42 kDa.
Actual Band: 35kDa (non-reduced); 40kDa (reduced)
|IHC-Fr||Use a concentration of 1 - 2 µg/ml.|
|ICC/IF||1/100 - 1/200.|
|IP||Use at 2-500 µg/mg of lysate.|
|Flow Cyt||Use 0.5-1µl for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionPlays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
Tissue specificityPresent only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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ab194401 has not yet been referenced specifically in any publications.