Anti-CD147 antibody (ab232991)
Key features and details
- Rabbit polyclonal to CD147
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-CD147 antibody
See all CD147 primary antibodies -
Description
Rabbit polyclonal to CD147 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Rat CD147 aa 50-350. (Expressed in E.coli).
Database link: P26453 -
Positive control
- WB: Recombinant rat CD147 protein; Rat liver lysate. IHC-P: Rat liver, pancreas, stomach and heart tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab232991 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 42 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 42 kDa. |
Target
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Function
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. -
Tissue specificity
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 25246 Rat
- SwissProt: P26453 Rat
- Unigene: 2269 Rat
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Alternative names
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
see all
Images
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Paraffin-embedded rat liver tissue stained for CD147 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded rat pancreas tissue stained for CD147 at 20μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded rat stomach tissue stained for CD147 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded rat heart tissue stained for CD147 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-CD147 antibody (ab232991) at 3 µg/ml + Recombinant rat CD147 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 42 kDa -
Anti-CD147 antibody (ab232991) at 3 µg/ml + Rat liver lysate
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232991 has not yet been referenced specifically in any publications.