RecombinantRabMAb

Recombinant Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
  • Flow Cytometry - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
  • Sandwich ELISA - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
  • Immunoprecipitation - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
  • Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18008-8] to CD147 - BSA and Azide free
  • Suitable for: Sandwich ELISA, WB, IHC-P, Flow Cyt, IP, ICC/IF
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD147 antibody [EPR18008-8] - BSA and Azide free
    See all CD147 primary antibodies

  • Description

    Rabbit monoclonal [EPR18008-8] to CD147 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Sandwich ELISA, WB, IHC-P, Flow Cyt, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment aa 100-350. The exact sequence is proprietary.
    Database link: P18572

  • Positive control

    • WB: Mouse CD147 recombinant protein fragment; RAW 264.7, WEHI-3 and bEND.3 whole cell lysates; mouse liver lysate. IHC-P: Mouse intestine tissue. ICC/IF: WEHI-231 and bEND.3 cells. Flow Cyt: Mouse thymocytes. IP: RAW 264.7 whole cell lysate.

  • General notes

    Ab222389 is the carrier-free version of ab188190. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab222389 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab222389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use an isotype control with this antibody.

 
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.

  • Tissue specificity

    Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    N-glycosylated.

  • Cellular localization

    Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • 5A11 antigen antibody
      • 5F7 antibody
      • BASI_HUMAN antibody
      • Basigin (Ok blood group) antibody
      • Basigin antibody
      • Blood brain barrier HT7 antigen antibody
      • Bsg antibody
      • CD 147 antibody
      • CD147 antibody
      • CD147 antigen antibody
      • Collagenase stimulatory factor antibody
      • EMMPRIN antibody
      • Extracellular matrix metalloproteinase inducer antibody
      • Leukocyte activation antigen M6 antibody
      • M 6 antibody
      • M6 antibody
      • M6 leukocyte activation antigen antibody
      • Neurothelin antibody
      • OK antibody
      • OK blood group antibody
      • OK blood group antigen antibody
      • TCSF antibody
      • Tumor cell derived collagenase stimulatory factor antibody
      • Tumor cell-derived collagenase stimulatory factor antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

      Immunohistochemical analysis of paraffin-embedded mouse intestine tissue labeling CD147 with ab188190 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on mouse intestine is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
      Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEND.3 (Mouse brain capillary endothelial cell line) cells labeling CD147 with ab188190 at 1/250 dilution, followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cell membrane staining on bEND.3 cell line. The nuclear counter stain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab188190 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
      -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

    • Flow Cytometry - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
      Flow Cytometry - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

      Flow cytometric analysis of fresh mouse thymocytes labeling CD147 with ab188190 at 1/200 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

    • Sandwich ELISA - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
      Sandwich ELISA - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

      Standard Curve for CD147 (Analyte: Recombinant mouse CD147 protein) dilution range 0 pg/mL to 500 pg/mL using Capture antibody at 0.2 ug/mL and Detector Antibody at 0.5 ug/mL. Secondary antibody: Peroxidase Streptavidin SA-HRP at 1/20000 dilution. Concentration of ab188190 may vary from lot to lot; please use this curve as guideline.

      Washing buffer: 1X PBST
      Blocking/Diluting buffer and concentration: 1% BSA/PBS

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
      Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (Mouse B Cell Lymphoma cell line) cells labeling CD147 with ab188190 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cell membrane staining on WEHI-231 cell line. The nuclear counter stain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab188190 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
      -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

    • Immunoprecipitation - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
      Immunoprecipitation - Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

      CD147 was immunoprecipitated from 1 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab188190 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab188190 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: RAW 264.7 whole cell lysate 10µg (Input).

      Lane 2: ab188190 IP in RAW 264.7 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188190 in RAW 264.7 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188190).

    • Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)
      Anti-CD147 antibody [EPR18008-8] - BSA and Azide free (ab222389)

    References (0)

    Publishing research using ab222389? Please let us know so that we can cite the reference in this datasheet.

    ab222389 has not yet been referenced specifically in any publications.

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