Overview

  • Product name

    Anti-CD147 antibody [MEM-M6/1] (FITC)
    See all CD147 primary antibodies
  • Description

    Mouse monoclonal [MEM-M6/1] to CD147 (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    ab69771 recognizes an epitope in the N-terminal Ig domain (D1) of CD147 and is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Protein A chromatography purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimmer).

  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Properties

Applications

Our Abpromise guarantee covers the use of ab69771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 20µl for 106 cells or 100µl whole blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
    • Tissue specificity

      Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5A11 antigen antibody
      • 5F7 antibody
      • BASI_HUMAN antibody
      • Basigin (Ok blood group) antibody
      • Basigin antibody
      • Blood brain barrier HT7 antigen antibody
      • Bsg antibody
      • CD 147 antibody
      • CD147 antibody
      • CD147 antigen antibody
      • Collagenase stimulatory factor antibody
      • EMMPRIN antibody
      • Extracellular matrix metalloproteinase inducer antibody
      • Leukocyte activation antigen M6 antibody
      • M 6 antibody
      • M6 antibody
      • M6 leukocyte activation antigen antibody
      • Neurothelin antibody
      • OK antibody
      • OK blood group antibody
      • OK blood group antigen antibody
      • TCSF antibody
      • Tumor cell derived collagenase stimulatory factor antibody
      • Tumor cell-derived collagenase stimulatory factor antibody
      see all

    Images

    • Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) FITC.

    References

    This product has been referenced in:

    • Calabro SR  et al. Hepatocyte produced matrix metalloproteinases are regulated by CD147 in liver fibrogenesis. PLoS One 9:e90571 (2014). Read more (PubMed: 25076423) »
    • Koch C  et al. T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol 11:777-86 (1999). Read more (PubMed: 10330283) »
    See all 2 Publications for this product

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