Western blot abreview for Anti-CD147 antibody [MEM-M6/2]

Good
Abreviews
Application
Western blot
Sample
Pig Cell lysate - whole cell (brain endothelial cells)
Gel Running Conditions
Reduced Denaturing (4-12 %)
Loading amount
1.7 µg
Specification
brain endothelial cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS pH 7.6 + 0.1 % Tween 20
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Goat Anti-mouse IgG, HRP-linked Antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 43 kDa

Additional data

Additional Notes
Genscript ExpressPlus™ PAGE Gel,10¿8, 4-12%. Lysis buffer with Complete protease inhibitor. Used dry blotting.

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Submitted Mar 23 2017

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