Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (ab119114)


  • Product name

    Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free
    See all CD147 primary antibodies
  • Description

    Mouse monoclonal [MEM-M6/6] to CD147 - Low endotoxin, Azide free
  • Host species

  • Specificity

    ab119114 recognizes Ig domain D2 (membrane proximal) of CD147(Neurothelin).
  • Tested applications

    Suitable for: ICC/IF, WB, Functional Studies, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Protein A-CR purified soluble recombinant Human CD147 (consisting of the CDNA encoding the hinge region, CH2-and CH3 domain of Human IgG1).

  • Positive control

    • 293 Human fibroblastoid cell line.
  • General notes

    Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.



Our Abpromise guarantee covers the use of ab119114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 42 kDa.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function

    Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
  • Tissue specificity

    Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational

  • Cellular localization

    Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5A11 antigen antibody
    • 5F7 antibody
    • BASI_HUMAN antibody
    • Basigin (Ok blood group) antibody
    • Basigin antibody
    • Blood brain barrier HT7 antigen antibody
    • Bsg antibody
    • CD 147 antibody
    • CD147 antibody
    • CD147 antigen antibody
    • Collagenase stimulatory factor antibody
    • EMMPRIN antibody
    • Extracellular matrix metalloproteinase inducer antibody
    • Leukocyte activation antigen M6 antibody
    • M 6 antibody
    • M6 antibody
    • M6 leukocyte activation antigen antibody
    • Neurothelin antibody
    • OK antibody
    • OK blood group antibody
    • OK blood group antigen antibody
    • TCSF antibody
    • Tumor cell derived collagenase stimulatory factor antibody
    • Tumor cell-derived collagenase stimulatory factor antibody
    see all


  • Human peripheral blood lymphocytes stained with ab119114 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab119114, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • ICC/IF image of ab119114 stained SV40 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119114, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Awandare GA  et al. Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture. Sci Rep 8:5782 (2018). Read more (PubMed: 29636510) »
  • Sharma S  et al. Genome-scale identification of cellular pathways required for cell surface recognition. Genome Res 28:1372-1382 (2018). Read more (PubMed: 29914970) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for contacting us.

The following paper describes production and characterization of the MEM-M6/6 clone, apparently by the same laboratory that wrote the 2003 paper from which you obtained your protocol.

Koch C. et al. T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol. 1999 May;11(5):777-86. PMID:10330283

Inhibition of T-cell activation is described in the last section of the results:

"Of the 15 CD147 mAb tested, mAb MEM-M6/6, recognizing a unique epitope, inhibited the OKT3-induced T cell proliferation up to 80% (Fig. 9A) when the mAb was added at a concentration of 0.1 μg/ml. Increasing and decreasing the concentration of MEM-M6/6 gradually abrogated the inhibitory effect."

As I mentioned in our conversation, we do not test T-cell inhibition in-house. The listing of "Functional Assays" in the Activities section of our datasheet is based on the data reported in this paper. I hope this information is helpful to you.

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Thank you for contacting Abcam regarding these antibodies. I have spoken with our laboratory regarding the light chain isotypes of ab666 and ab119114. Unfortunately this information is not available. Please do not hesitate to contact us if there is anything else that we may do to help you reach your research goals. 

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Vielen Dank für Ihre Anfrage. Leider haben wir keinen JAM-A (Junctional Adhesion Molecule 1), der auch für funktionale Studien getestet ist. Ich möchte Ihnen deshalb die Webseite Biocompare empfehlen. Für EMMPRIN (CD147) haben wir einen Antikörper, der für funktionale Studien getestet ist und der die CD3 induzierte Aktivierung von T Zellen hemmt. Wenn ich Sie richtig verstanden habe, ist das allerdings genau falschherum für Ihre geplanten Experimente? Click here (or use the following: ab119114 erkennt die Ig Domäne D2 von CD147, ein 50-60kDa großes Typ I Transmembran-Glykoprotein, dass vor allem von Leukozyten, Erythrozyten, Platelets und Endothelzellen exprimiert wird. Es wird nicht von "resting" Lymphozyten exprimiert. Falls Sie einen Antikörper suchen, der den negativen Rezeptor CD147 blockiert, muss ich Ihnen leider ebenfalls mitteilen, dass wir so einen Antikörper leider ebenfalls nicht anbieten. Es tut mir leid, dass ich Ihnen in diesem Fall keine positive Antwort geben kann und hoffe, dass diese Information dennoch hilfreich ist.

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