Key features and details
- Mouse monoclonal [OX47] to CD147
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Rat
- Isotype: IgG1
Product nameAnti-CD147 antibody [OX47]
See all CD147 primary antibodies
DescriptionMouse monoclonal [OX47] to CD147
Tested applicationsSuitable for: Flow Cyt, IHC-Frmore details
Species reactivityReacts with: Rat
Tissue, cells or virus corresponding to CD147. T lymphocytes from a mixed lymphocyte reaction between purified rat T helper cells and irradiated semiallogeneic rat spleen
- IHC-Fr: Rat spleen tissue. Flow Cyt: Lewis rat splenocytes.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab238462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 5 µg/ml.|
FunctionPlays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
Tissue specificityPresent only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
ab150117) was used at 1/2000 for 30 min on ice.
ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
IHC image of CD147 staining in a section of frozen normal rat colon*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab238462 (shown in green) at 5g/ml.
The section was then incubated with ab150117 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488, 1/1000)) presabsorbed for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
*Tissue obtained from Charles River.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238462 has not yet been referenced specifically in any publications.