Overview

  • Product name

    Anti-CD147 antibody [OX47] - BSA and Azide free
    See all CD147 primary antibodies
  • Description

    Mouse monoclonal [OX47] to CD147 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to CD147. T lymphocytes from a mixed lymphocyte reaction between purified rat T helper cells and irradiated semiallogeneic rat spleen

  • Positive control

    • IHC-Fr: Rat spleen. FC: Lewis rat splenocytes.
  • General notes

    ab244578 is a PBS only version of ab238462.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab244578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use a concentration of 5 µg/ml.

Target

  • Function

    Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
  • Tissue specificity

    Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5A11 antigen antibody
    • 5F7 antibody
    • BASI_HUMAN antibody
    • Basigin (Ok blood group) antibody
    • Basigin antibody
    • Blood brain barrier HT7 antigen antibody
    • Bsg antibody
    • CD 147 antibody
    • CD147 antibody
    • CD147 antigen antibody
    • Collagenase stimulatory factor antibody
    • EMMPRIN antibody
    • Extracellular matrix metalloproteinase inducer antibody
    • Leukocyte activation antigen M6 antibody
    • M 6 antibody
    • M6 antibody
    • M6 leukocyte activation antigen antibody
    • Neurothelin antibody
    • OK antibody
    • OK blood group antibody
    • OK blood group antigen antibody
    • TCSF antibody
    • Tumor cell derived collagenase stimulatory factor antibody
    • Tumor cell-derived collagenase stimulatory factor antibody
    see all

Images

  • ab238462)ab238462 (red line). The cells were incubated in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab238462) (1xab150117) was used at 1/2000 for 30 min on ice.ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  • This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab238462).

    IHC image of CD147 staining in a section of frozen normal rat colon*.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab238462 (shown in green) at 5g/ml.

    The section was then incubated with ab150117 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488, 1/1000)) presabsorbed for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

    *Tissue obtained from Charles River.

References

ab244578 has not yet been referenced specifically in any publications.

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