Overview

  • Product name

    Anti-CD15 antibody [FUT4/815] - BSA and Azide free
    See all CD15 primary antibodies
  • Description

    Mouse monoclonal [FUT4/815] to CD15 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Tissue, cells or virus corresponding to Human CD15. Purified human neutrophils

  • Positive control

    • U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab212396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

Target

  • Relevance

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Alternative names

    • 3 fucosyl N acetyl lactosamine antibody
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed paraffin-embedded section of human Hodgkin's lymphoma tissue labeling FUT4 with ab212396 at 1μg/mL.

References

This product has been referenced in:

  • Nan J  et al. Endoplasmic reticulum stress induced LOX-1+  CD15+ polymorphonuclear myeloid-derived suppressor cells in hepatocellular carcinoma. Immunology 154:144-155 (2018). IHC-P ; Human . Read more (PubMed: 29211299) »
See 1 Publication for this product

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