Overview

  • Product name
    Anti-CD15 antibody [HI98]
    See all CD15 primary antibodies
  • Description
    Mouse monoclonal [HI98] to CD15
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Full length protein corresponding to Human CD15.

  • Positive control
    • Human uterus tissue; Human peripheral blood granulocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab188610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab18400 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization
    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Alternative names
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human uterus tissue labeling CD15 with ab188610 at 1/50 dilution.

  • Flow cytometric analysis of Human peripheral blood granulocytes (right histogram) labeling CD15 with ab188610.  FITC-conjugated anti-mouse secondary antibodies were used for the analysis.

References

ab188610 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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