Overview

  • Product name

    Anti-CD15 antibody [Leu-M1]
    See all CD15 primary antibodies
  • Description

    Mouse monoclonal [Leu-M1] to CD15
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Tissue, cells or virus corresponding to Human CD15. (U937 histiocytic cell line).

  • Positive control

    • IHC-P: Human Hodgkin's lymphoma tissue. Flow Cyt: Human monocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab233867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at RT.

Target

  • Relevance

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Alternative names

    • 3 fucosyl N acetyl lactosamine antibody
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for CD15 using ab233867 at 1.0 µg/ml in immunohistochemical analysis.

  • Flow cytometric analysis of human monocytes labeling CD15 with ab233867 at 1 µg/106 cells (red).

References

ab233867 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233867.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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