Key features and details
- Mouse monoclonal [Leu-M1] to CD15
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Species independent
- Isotype: IgM
Product nameAnti-CD15 antibody [Leu-M1]
See all CD15 primary antibodies
DescriptionMouse monoclonal [Leu-M1] to CD15
Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Species independent
Tissue, cells or virus corresponding to Human CD15. (U937 histiocytic cell line).
- IHC-P: Human Hodgkin's lymphoma tissue. Flow Cyt: Human monocytes.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
Purification notesPurified from Bioreactor Concentrate.
Light chain typekappa
Our Abpromise guarantee covers the use of ab233867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5-1µg for 106 cells.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at RT.
RelevanceCD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
Cellular localizationGolgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233867 has not yet been referenced specifically in any publications.