Anti-CD15 antibody [Leu-M1] - BSA and Azide free (ab212391)

Mouse monoclonal CD15 antibody [Leu-M1]. Validated in IHC, Flow Cyt, ICC/IF and tested in Human. Immunogen corresponding to tissue, cells or virus.

Overview

  • Product name

    Anti-CD15 antibody [Leu-M1] - BSA and Azide free
    See all CD15 primary antibodies
  • Description

    Mouse monoclonal [Leu-M1] to CD15 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Tissue, cells or virus corresponding to Human CD15. U937 histiocytic cell line

  • Positive control

    • U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma

Properties

Applications

Our Abpromise guarantee covers the use of ab212391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

for 30 minutes at room temperature.

Target

  • Relevance

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Alternative names

    • 3 fucosyl N acetyl lactosamine antibody
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue section labeling FUT4 with ab212391 at 1µg/mL.

  • Flow cytometric analysis of Human monocytes labeling FUT4 with ab212391.

References

ab212391 has not yet been referenced specifically in any publications.

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