Key features and details
- Mouse monoclonal [Leu-M1] to CD15 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Species independent
- Isotype: IgM
Product nameAnti-CD15 antibody [Leu-M1] - BSA and Azide free
See all CD15 primary antibodies
DescriptionMouse monoclonal [Leu-M1] to CD15 - BSA and Azide free
Tested applicationsSuitable for: IHC-P, Flow Cytmore details
Species reactivityReacts with: Species independent
Tissue, cells or virus corresponding to Human CD15. (U937 histiocytic cell line).
- IHC-P: Human Hodgkin's lymphoma tissue.Flow Cyt: Human monocytes.
ab269778 is a carrier free version of ab233867. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
Purification notesPurified from Bioreactor Concentrate.
Light chain typekappa
Our Abpromise guarantee covers the use of ab269778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at RT.
|Flow Cyt||Use 0.5-1µg for 10 cells.|
RelevanceCD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
Cellular localizationGolgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab269778 has not yet been referenced specifically in any publications.