Overview

  • Product name

    Anti-CD15 antibody [MCS-1], prediluted (CF405M)
    See all CD15 primary antibodies
  • Description

    Mouse monoclonal [MCS-1] to CD15, prediluted (CF405M)
  • Host species

    Mouse
  • Conjugation

    CF405M. Ex: 408nm, Em: 452nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    White blood cells

  • Positive control

    • Normal Human Peripheral Blood
  • General notes

    CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab123633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells.

ab126029 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Alternative names

    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Images

  • Flow cytometry analysis of CD15 expression in Normal Peripheral Blood from a Human Donor using ab123633 at 5 µl/106 cells.
  • Flow cytometry analysis of CD15 expression in Normal Peripheral Blood from a Human Donor using ab123633 at 5 µl/106 cells.

References

ab123633 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Flow Cytometry
Fixation
None
Permeabilization
No
Sample
Sheep Cell (Peripheral blood)
Specification
Peripheral blood
Gating Strategy
Granulocytes
Preparation
Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None

Ms. Ina Laura Pieper

Verified customer

Submitted Sep 16 2013

Application
Flow Cytometry
Fixation
None
Permeabilization
No
Sample
Cow Cell (Peripheral blood)
Specification
Peripheral blood
Gating Strategy
Granulocytes
Preparation
Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None

Ms. Ina Laura Pieper

Verified customer

Submitted Sep 16 2013

Application
Flow Cytometry
Fixation
None
Permeabilization
No
Sample
Human Cell (Peripheral blood)
Specification
Peripheral blood
Gating Strategy
Granulocytes
Preparation
Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None

Ms. Ina Laura Pieper

Verified customer

Submitted Sep 11 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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