Anti-CD15 antibody [MEM-158] (ab665)
![Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)](https://www.abcam.com/ps/products/0/ab665/Images/ab665-2-anti-cd15-antibody-mem-158-flow-cytometry-histogram-peripheral-blood-human.jpg "Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)")
Key features and details
- Mouse monoclonal [MEM-158] to CD15
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgM
Related conjugates and formulations
Overview
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Product name
Anti-CD15 antibody [MEM-158]
See all CD15 primary antibodies -
Description
Mouse monoclonal [MEM-158] to CD15 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD15. Human granulocytes
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Positive control
- Flow Cyt: Human peripheral whole blood.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.097% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-158 -
Isotype
IgM -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use a concentration of 1 - 4 µg/ml. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
Cellular localization
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
Alternative names
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
Images
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Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)
Flow Cytometry analysis of human peripheral whole blood labeling CD15 (surface staining) with ab665 at 0.3 µg/mL. CD15 positive human neutrophil granulocytes (red-filled). CD15 negative lymphocytes (black-dashed).
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![Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)](https://www.abcam.com/ps/products/0/ab665/Images/ab665-1-anti-cd15-antibody-mem-158-flow-cytometry-scatter-plot-peripheral-blood-human.jpg)
Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)Flow cytometry surface staining pattern of human peripheral blood stained using Anti-CD15 antibody [MEM-158] (ab665) at 0.3 µg/mL.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab665 has been referenced in 1 publication.
- Kishimoto T. (Eds.) et al. Leucocyte typing VI. Garland Publishing Inc. : (1997). PubMed: