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    cd15-antibody-mem-158-ab665.pdf

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Anti-CD15 antibody [MEM-158] (ab665)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)
  • Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)

Key features and details

  • Mouse monoclonal [MEM-158] to CD15
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgM

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Overview

  • Product name

    Anti-CD15 antibody [MEM-158]
    See all CD15 primary antibodies
  • Description

    Mouse monoclonal [MEM-158] to CD15
  • Host species

    Mouse
  • Specificity

    Human CD15 antigen.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human granulocytes).

  • Positive control

    • Flow Cyt: Human peripheral whole blood.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.00
    Preservative: 0.097% Sodium azide
    Constituent: Tris buffered saline
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    MEM-158
  • Isotype

    IgM
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Cancer
    • Tumor immunology
    • CD markers
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers

Associated products

  • Alternative Versions

    • FITC Anti-CD15 antibody [MEM-158] (ab18272)
    • PerCP/Cy5.5® Anti-CD15 antibody [MEM-158] (ab234266)
    • Biotin Anti-CD15 antibody [MEM-158] (ab28103)
    • APC Anti-CD15 antibody [MEM-158] (ab88032)
  • Compatible Secondaries

    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use a concentration of 1 - 4 µg/ml.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Notes
Flow Cyt
Use a concentration of 1 - 4 µg/ml.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Alternative names

    • 3 fucosyl N acetyl lactosamine antibody
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Images

  • Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)
    Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)

    Flow Cytometry analysis of human peripheral whole blood labeling CD15 (surface staining) with ab665 at 0.3 µg/mL. CD15 positive human neutrophil granulocytes (red-filled). CD15 negative lymphocytes (black-dashed).

  • Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)
    Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)
    Flow cytometry surface staining pattern of human peripheral blood stained using Anti-CD15 antibody [MEM-158] (ab665) at 0.3 µg/mL.

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab665? Please let us know so that we can cite the reference in this datasheet.

ab665 has been referenced in 1 publication.

  • Kishimoto T. (Eds.) et al. Leucocyte typing VI. Garland Publishing Inc. : (1997). PubMed:

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-CD15 antibody [MEM-158]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cord blood)
Specification
Cord blood
Fixative
Methanol
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10%
Read More

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted May 25 2011

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