Anti-CD15 antibody [MEM-158] (ab665)
![Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)](https://www.abcam.com/ps/products/0/ab665/Images/ab665-2-anti-cd15-antibody-mem-158-flow-cytometry-histogram-peripheral-blood-human.jpg "Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)")
Key features and details
- Mouse monoclonal [MEM-158] to CD15
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-CD15 antibody [MEM-158]
See all CD15 primary antibodies -
Description
Mouse monoclonal [MEM-158] to CD15 -
Host species
Mouse -
Specificity
Human CD15 antigen. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue/ cell preparation (Human granulocytes).
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Positive control
- Flow Cyt: Human peripheral whole blood.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.097% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-158 -
Isotype
IgM -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use a concentration of 1 - 4 µg/ml. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
Cellular localization
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
Alternative names
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
Images
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Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)
Flow Cytometry analysis of human peripheral whole blood labeling CD15 (surface staining) with ab665 at 0.3 µg/mL. CD15 positive human neutrophil granulocytes (red-filled). CD15 negative lymphocytes (black-dashed).
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![Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)](https://www.abcam.com/ps/products/0/ab665/Images/ab665-1-anti-cd15-antibody-mem-158-flow-cytometry-scatter-plot-peripheral-blood-human.jpg)
Flow Cytometry - Anti-CD15 antibody [MEM-158] (ab665)Flow cytometry surface staining pattern of human peripheral blood stained using Anti-CD15 antibody [MEM-158] (ab665) at 0.3 µg/mL.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab665 has been referenced in 1 publication.
- Kishimoto T. (Eds.) et al. Leucocyte typing VI. Garland Publishing Inc. : (1997). PubMed: