Overview

  • Product name

    Anti-CD15 antibody [MMA]
    See all CD15 primary antibodies
  • Description

    Mouse monoclonal [MMA] to CD15
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type corresponding to CD15. BALB/C mice were injected with U937 histiocytic cell line.

  • Positive control

    • IHC-P: Human Hodgkin Lymphoma. Flow Cytometry: Human whole blood
  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab17080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Alternative names

    • 3 fucosyl N acetyl lactosamine antibody
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Images

  • IHC image of CD15 staining in Human tonsil formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). The section was incubated with ab17080, 1µg/ml, for 15 mins at room temperature.  A Goat polyclonal Secondary Antibody to Mouse IgM secondary antibody (ab97230) was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Human whole blood stained with ab17080 (right) or mouse IgM isotype control ab91545 (left). Red blood cells of 200ul human whole blood were lysed, then cells were incubated for 30 min on ice in 1x PBS containing 10ug/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab17080 ) or IgM isotype control (ab91545) (100ul at 1ug/ml) for 30 min on ice.

    The secondary antibody Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (ab150121) was used at 1/2000 dilution for 30 min at 4° C. Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were collected with the forward and side light-scatter characteristics of viable cells.

References

ab17080 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your patience.

I have received an update from the lab and unfortunately they are unable to conjugate this antibody with fluorophores. Therefore the conjugation kits we provide would be the most suitable alternative. If you have any further questions just let me know.

Apologies for any inconvenience.

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Answer

Thank you for your patience.

I am still waiting for the lab to confirm whether it will be possible to conjugate the clone MMA (Anti-CD15 antibody) to APC, APC/Cy7 or Alexa fluor 647.

We have a number of conjugation kits that enable you to conjugate antibodies with APC/Cy7. Here are the links to the products.

ab102856 EasyLink APC/Cy7® Conjugation Kit (1 x 1mg APC/Cy7®)
www.abcam.com/ab102856

ab102857 EasyLink APC/Cy7® Conjugation Kit (1x100µg APC/Cy7®)
www.abcam.com/ab102857

ab102858 EasyLink APC/Cy7® Conjugation Kit (3x100µg APC/Cy7®)
www.abcam.com/ab102858

ab102859 EasyLink APC/Cy7® Conjugation Kit (3 x 10µg APC/Cy7®)
www.abcam.com/ab102859

I will provide further information about purchasing already conjugated antibodies as soon as I receive information from the lab.

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Question
Answer

Thank you for your enquiry. The concentration of this antibody is 100 ug/ml. Please do not hesitate to contact us if you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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